Basic Concepts of Molecular Pathology
A.Y. 2023/2024
Learning objectives
The course aims to provide students with instruments to understand the molecular bases of pathological disorders and practical skills related to molecular methodologies for diagnosis and research for the study of animal diseases.
Lectures focus on the most common molecular techniques and their application to the study and diagnosis of diseases.
During practical classes, students will acquire skills in molecular techniques for the study of proteins (Western blot, immunohistochemistry, flow cytometry) and nucleic acids (PCR and RT-PCR).
Lectures focus on the most common molecular techniques and their application to the study and diagnosis of diseases.
During practical classes, students will acquire skills in molecular techniques for the study of proteins (Western blot, immunohistochemistry, flow cytometry) and nucleic acids (PCR and RT-PCR).
Expected learning outcomes
At the end of the course, the student will acquire the fundamental knowledge of the molecular mechanisms of disorders and the basic techniques to investigate them.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
The course will provide theoretical and practical knowledge of basic biomolecular methodologies useful for diagnostics and research for the study of animal diseases. During the theoretical hours, lectures are provided on the most common biomolecular techniques and their application to the study and diagnosis of diseases of companion animals and livestock.
Practicals
· Protein purification from pathological tissues and western blot
· RNA extraction and PCR for evaluating the expression of genes involved in physiopathological processes
· Morphology, histology, and immunohistochemistry in veterinary oncology
· PBMC purification from peripheral whole blood and cell viability evaluation
Practicals
· Protein purification from pathological tissues and western blot
· RNA extraction and PCR for evaluating the expression of genes involved in physiopathological processes
· Morphology, histology, and immunohistochemistry in veterinary oncology
· PBMC purification from peripheral whole blood and cell viability evaluation
Prerequisites for admission
Recommended: basic knowledge of general pathology, biochemistry, and immunology.
Teaching methods
Frontal teaching, Seminars, and Practicals in the laboratory
Teaching Resources
Teaching material will be posted on the ARIEL platform. Exam questions will be extracted only from the teaching material made available.
Consultation text: Robbin's and Cotran's Pathologic basis of Disease only to be considered as a suggestion for those students who would like to deepen their knowledge in general and special pathology. This book is a human pathology book.
Consultation text: Robbin's and Cotran's Pathologic basis of Disease only to be considered as a suggestion for those students who would like to deepen their knowledge in general and special pathology. This book is a human pathology book.
Assessment methods and Criteria
The exam will be written and will consist of multiple choice questions (majority) and other formats such as fill-in-the-table, lists, and short essay-type questions (minority).
Enrollment by SIFA is mandatory to access the exam.
Enrollment by SIFA is mandatory to access the exam.
VET/03 - VETERINARY PATHOLOGY - University credits: 3
Practicals: 16 hours
Lessons: 16 hours
Lessons: 16 hours
Professor:
Lecchi Cristina
Educational website(s)
Professor(s)
Reception:
upon appointment
Via dell'Università 6, Lodi