General Microbiology
A.Y. 2023/2024
Learning objectives
The course aims to provide students with basic microbiology knowledge, and the general information necessary for the application of microbiological methodologies to biotechnology. The course focuses on the analysis of microbial cellular structure, physiology and genetics, on potential applications of microorganisms in biotechnology and on the mechanisms of pathogenesis by both bacteria and viruses.
Expected learning outcomes
The students will acquire detailed knowledge and understanding of the composition and fundamental biological processes of bacterial cells, and will achieve a clear understanding of microbial diversity at the level of metabolic processes and gene regulation.
The student will be able to use basic methods of microbiology and apply the cultural skills acquired in advanced courses.
Critical and judgmental skills, and ability of students to communicate what they have learned, are mostly developed through laboratory activities and discussion of the results obtained.
The microbiological culture acquired through this course will provide the student with the skills to study microbiology even in a completely autonomous way.
The student will be able to use basic methods of microbiology and apply the cultural skills acquired in advanced courses.
Critical and judgmental skills, and ability of students to communicate what they have learned, are mostly developed through laboratory activities and discussion of the results obtained.
The microbiological culture acquired through this course will provide the student with the skills to study microbiology even in a completely autonomous way.
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Linea AL
Responsible
Lesson period
First semester
Course syllabus
Characteristics of prokaryotic and eukaryotic microorganisms, morphology of bacteria and eumycetes
Nutritional requisites of prokaryotes and culture methods; identification with 16S rRNA and molecular taxonomy
Bacterial growth and direct / indirect methods for assessing growth
Growth control: antibiotics
Cytology: membrane, cell wall, cytoplasm
Structural and biochemical differences between Bacteria / Archea
Structural and biochemical differences between Gram positive and Gram negative bacteria
Sporulation
The fundamental biological processes in Bacteria: DNA replication, transcription, translation cell wall biosynthesis
Mechanisms of gene regulation in response to nutritive availability: cAMP, ppGpp
Two-component regulation systems: regulation of flagellar motility
Microbial genetics: gene transfer: conjugation transformation, mobile genetic elements, transduction
Elements of virology: bacteriophages, examples of animal viruses (retrovirus, RNA viruses), mechanisms of infection and interaction with host cells
Antibiotics: mechanisms of action and resistance
Microbial metabolism: aerobic respiration, anaerobic respiration, fermentation
Oxygenic and anoxic photosynthesis, assimilation of CO2
Nitrogen Fixing
Mycete classification, life cycle, nutrition
Biogeochemical cycles (C, N, P, S)
Laboratory activities (1CFU = 16 HOURS)
Plating and isolation of pure cultures of microorganisms. Selective growth conditions (anaerobiosis, minimal and differential terrain). Growth curves and antibiotic sensitivity. Microscopy: observation of live cells, Gram staining. Antibiotic susceptibility tests.
Nutritional requisites of prokaryotes and culture methods; identification with 16S rRNA and molecular taxonomy
Bacterial growth and direct / indirect methods for assessing growth
Growth control: antibiotics
Cytology: membrane, cell wall, cytoplasm
Structural and biochemical differences between Bacteria / Archea
Structural and biochemical differences between Gram positive and Gram negative bacteria
Sporulation
The fundamental biological processes in Bacteria: DNA replication, transcription, translation cell wall biosynthesis
Mechanisms of gene regulation in response to nutritive availability: cAMP, ppGpp
Two-component regulation systems: regulation of flagellar motility
Microbial genetics: gene transfer: conjugation transformation, mobile genetic elements, transduction
Elements of virology: bacteriophages, examples of animal viruses (retrovirus, RNA viruses), mechanisms of infection and interaction with host cells
Antibiotics: mechanisms of action and resistance
Microbial metabolism: aerobic respiration, anaerobic respiration, fermentation
Oxygenic and anoxic photosynthesis, assimilation of CO2
Nitrogen Fixing
Mycete classification, life cycle, nutrition
Biogeochemical cycles (C, N, P, S)
Laboratory activities (1CFU = 16 HOURS)
Plating and isolation of pure cultures of microorganisms. Selective growth conditions (anaerobiosis, minimal and differential terrain). Growth curves and antibiotic sensitivity. Microscopy: observation of live cells, Gram staining. Antibiotic susceptibility tests.
Prerequisites for admission
Good basic knowledge of General Biology, Genetics
Teaching methods
frontal lectures (see programme below for details)
Laboratory classes (see programme below for details)
Laboratory classes (see programme below for details)
Teaching Resources
Textbook: Dehò G. e Galli E. Biologia dei microrganismi. Casa Editrice Ambrosiana
Lecture powerpoint presentations, and recordings of lectures from academic year 2020/21 are available on the instructor's Ariel website
Lecture powerpoint presentations, and recordings of lectures from academic year 2020/21 are available on the instructor's Ariel website
Assessment methods and Criteria
The final exam is structured as follows:
1) Verification of the ability to apply the basic knowledge acquired in the laboratory classes (drawing a bacterial growth curve on semilogarithmic paper).
2) Two open questions aimed at testing the knowledge of general concepts discussed in the lectures (e.g., basic biological processes in bacteria, gene regulation, energy metabolism).
3) Four open questions concerning more specific aspects discussed in the lectures
1) Verification of the ability to apply the basic knowledge acquired in the laboratory classes (drawing a bacterial growth curve on semilogarithmic paper).
2) Two open questions aimed at testing the knowledge of general concepts discussed in the lectures (e.g., basic biological processes in bacteria, gene regulation, energy metabolism).
3) Four open questions concerning more specific aspects discussed in the lectures
BIO/19 - MICROBIOLOGY - University credits: 9
Single bench laboratory practical: 16 hours
Lessons: 64 hours
Lessons: 64 hours
Professors:
Landini Paolo, Rossi Elio
Linea MZ
Responsible
Lesson period
First semester
Course syllabus
Prokaryote/eukaryote difference, morphology of bacteria and mycetes
Nutritional categories, cultural methods, methods for microbial identification and molecular taxonomy
Bacterial growth, direct/indirect evaluation methods
Growth control: antibiotics, action and resistance mechanisms
Prokaryote cytology: membrane, wall, cytoplasm; Bacteria/archaea; gram positive/negative
Spore formers and sporogenesis
Bacterial genetic material, DNA replication, mutations, natural selection, mutagens
Fundamental prokaryote processes: transcription, traduction, duplication
Mechanisms of gene regulation
bacterial genetics: horizontal gene exchange, transformation, conjugation, mobile genetic elements, transduction
virology: bacteriophages, interaction with host cell
microbial metabolism: aerobic and anaerobic respiration, fermentation, chemolitotrophy
oxygenic and anoxygenic photosynthesis, metabolism of CO2 fixation
nitrogen fixation
Mycetes: classification, growth cycle, nutrition
Element cycles (C, N, P, S)
Symbiosis between microorganisms and between microorganism and eukaryotic hosts
Nutritional categories, cultural methods, methods for microbial identification and molecular taxonomy
Bacterial growth, direct/indirect evaluation methods
Growth control: antibiotics, action and resistance mechanisms
Prokaryote cytology: membrane, wall, cytoplasm; Bacteria/archaea; gram positive/negative
Spore formers and sporogenesis
Bacterial genetic material, DNA replication, mutations, natural selection, mutagens
Fundamental prokaryote processes: transcription, traduction, duplication
Mechanisms of gene regulation
bacterial genetics: horizontal gene exchange, transformation, conjugation, mobile genetic elements, transduction
virology: bacteriophages, interaction with host cell
microbial metabolism: aerobic and anaerobic respiration, fermentation, chemolitotrophy
oxygenic and anoxygenic photosynthesis, metabolism of CO2 fixation
nitrogen fixation
Mycetes: classification, growth cycle, nutrition
Element cycles (C, N, P, S)
Symbiosis between microorganisms and between microorganism and eukaryotic hosts
Prerequisites for admission
Basic knowledge in general and cellular biology; organic and inorganic chemistry
Teaching methods
The course is based on 32 lesson (64 hours) where will be described the main topics of the general microbiology and practical activities (16 hours) i) in laboratory, where the students will acquire knowledge about safety in the biological laboratory and the basic techniques for microbial count, identification and characterisation and ii) class-exercises and autonomous work to analyse nucleotide sequence, bacterial growth, bibliographic data
Teaching Resources
Dehò G. e Galli E. Biologia dei microrganismi. Casa Editrice Ambrosiana
Slides showed during the lessons with video and bibliographic references to in-depth scientific articles will be available through the Ariel web portal
Slides showed during the lessons with video and bibliographic references to in-depth scientific articles will be available through the Ariel web portal
Assessment methods and Criteria
The final exam is written and based on the answer to 3 open questions on selected topics described during the lessons (theoretical and practical), finalised to evaluate the aquired knowledge on general microbiology. The use of a correct scientific language will be part of the exam evaluation.
If requested before the exam date, the exam can be given in the English language.
A minimum number of 7 exam dates will be published in SIFA.
If requested before the exam date, the exam can be given in the English language.
A minimum number of 7 exam dates will be published in SIFA.
BIO/19 - MICROBIOLOGY - University credits: 9
Single bench laboratory practical: 16 hours
Lessons: 64 hours
Lessons: 64 hours
Professors:
Borin Sara, Rolli Eleonora
Educational website(s)
Professor(s)
Reception:
everyday under appointment
office: via Mangiagalli 25, 3rd floor, Environmental Microbiology Section
Reception:
On appointment by e-mail
Via Celoria 26, Scientific buildings, 4th floor, Tower "B"
Reception:
Contact for an appointment
Online