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Ree Crispres - a Workshop On Genome Editing Technologies

A.Y. 2023/2024
3
Max ECTS
40
Overall hours
SSD
AGR/03 AGR/07 AGR/12 BIO/01
Language
English
Learning objectives
This supplementary activity provides students with skills in the following areas:
· Identification of target genes for the protection of plants of agricultural interest
· Planning of experiments using genome editing
· Analysis of sequences resulting from genomic editing events
· In vitro cultures
· Phenotyping of the disease
During the pandemic emergency, workshops will be conducted online, based on e-learning and active learning platforms, including virtual labs and gamification.
Expected learning outcomes
The REE lab will provide 3 CFUs, for a total of 40 hours of class work. The work will be online, during the pandemic emergency. Lectures are provided on a e-learning platform and virtual labs are based on interactive, virtual experiences. The final approval is based on a project that the student independently prepares and presents to the class and the trainers. The REE lab is part of an international training project and is hosting students from two European Universities.

Lesson period: Second semester

Lessons timetable

Assessment methods: Giudizio di approvazione
Assessment result: superato/non superato

Exams calendar

Single course

This course can be attended as a single course.

Take a single course
Course syllabus and organization

Single session

Responsible
Pozzi Carlo Massimo
Lesson period
Second semester
Course syllabus
Day 1: Introduction and bioinformatics lab
Day 2: Gateway cloning; E. coli transformation; miniprep; inoculation
Day 3: Rice transformation and visit of mutants growing in the parcels
Day 4: Workshop sequence analysis
Day 5: Invited speaker; round table and Q&A.
A final event takes place in the fall.
Prerequisites for admission
No formal prerequisites. It is strongly advised to have a basic knowledge in molecular biology.
Good understanding of English is required.
Teaching methods
The workshop includes lectures and practical experiences. The practical experiences will take place in the wet lab and at the computer.
Teaching Resources
Plant Genome Editing with CRISPR system, methods and protocols. (2019) Y.Qi Editor, doi: 10.1007/978-1-4939-8991-1

The CRISPres Handbook (2021). Published by the CRISPres project.
Assessment methods and Criteria
The knowledge acquired by the students is evaluated through a final report, based on a given .doc format, where they describe a project autonomously developed. If a minimum set of competencies will not be reached, the student will not have the 3 ECTS granted. The competencies will be evaluated during the final discussion. The students have to present their project during a "knowledge challenge": teams of students prepare a project on a topic of choice and using the tools learned during the class. All the teams present their project and the teachers + peers will select the best one, based on explicit criteria.
Students with SLD may request for specifically adapted evaluation modalities.
AGR/03 - ARBORICULTURE AND FRUITCULTURE
AGR/07 - AGRICULTURAL GENETICS
AGR/12 - PLANT PATHOLOGY
BIO/01 - GENERAL BOTANY
Computer room practicals: 16 hours
Laboratories: 16 hours
Lessons: 8 hours
Professors: Brambilla Vittoria Francesca, Pozzi Carlo Massimo
Educational website(s)
Ree Crispres - A workshop on genome editing technologies (a.y. 2023/24)
Professor(s)