Biochemical, Biomolecular, and Chemical Approaches for Manipulating Biological Systems

The course aims to provide an in-depth understanding of the biochemical and genetic aspects of prokaryotic and eukaryotic cells, as well as cell culture and genome editing techniques; the biochemical and genetic aspects of model organisms used in research and cellular and molecular techniques for gene manipulation and regulation of gene expression, applicable to models of disease. Additionally, the course aims to provide knowledge related to the structure, function, and analysis of biological and synthetic macromolecules, such as oligonucleotides and analogues, as well as the cellular processes in which they are involved and their applications in modulating gene expression. Furthermore, it delves into the techniques of designing, synthesizing and modifying oligonucleotides and oligonucleotide analogues for their use in the manipulation of biological systems.

Upon completion of the course, students are expected to master their knowledge in biochemistry, genetics, and cell biology and the main methodologies used in molecular and cellular biotechnology, also applicable at designing therapies. Students should also be able to identify techniques and technologies for the design and generation of synthetic oligonucleotides to manipulate biological systems, aiming to develop advanced therapies. Furthermore, students should have skills related to the application of cell and animal models for the study of communicable and noncommunicable diseases and should be able to critically evaluate experimental design and validation approaches aimed at manipulating biological systems in physiological and pathological settings.