Approccio diagnostico e profilattico alle malattie infettive e trasmissibili dei pet

- Review of viral diagnostics (2 hours)
- Laboratory practical sessions for molecular diagnostics (viruses): preparation of classical and real-time pcr mix, dna and rna extraction from samples, reading and interpretation of classical and real-time pcr results, sequencing and phylogenetic analysis (8 hours)
- Analysis of the most important viral infectious diseases and emerging infectious zoonoses in dogs and cats: presentation of clinical cases evaluated together with students for proposing the best diagnostic and management approach (6 hours)
- Seminar on infectious diseases in non-conventional animals (4 hours)
- Educational visit to Istituto Zooprofilattico Sperimentale Lombardia-Emilia-Romagna, Brescia branch, for viral diagnostics (electron microscopy, viral isolation) (4 hours)
- Educational visit to Istituto Zooprofilattico Sperimentale Lombardia-Emilia-Romagna, Pavia section, for bls3 operations (4 hours)
- Educational visit to a dog and cat shelter in milan for management aspects and infectious disease prevention in dogs and cats (4 hours)
- Educational trip to the Lombardy-Emilia-Romagna Experimental Zooprophylactic Institute, Pavia branch, for BLS3 operation (4 hours)
- Educational trip to the Milan dog shelter for management aspects and prevention of infectious diseases in dogs and cats (4 hours)

- Principles of bacteriological diagnostics (4 hours)
- Sample selection and collection methods (4 hours)
- Bacteriological diagnostic techniques (laboratory practical sessions): preparation of culture media and inoculation of biological samples; bacterial isolation and identification using classical and innovative methods (maldi-tof); execution, reading, and interpretation of antibiograms and mic (8 hours)
- Isolation and identification of key microorganisms in pets (8 hours)
- Discussion of individual bacterial infections of interest in pets (4 hours)
o Leptospira spp.
o Bordetella spp.
o Staphylococcal infections
o Gram-negative bacterial infections
o Enteric bacterial infections
o Urinary tract infections (uti)
- Correct use of antibiotics and antibiotic resistance (4 hours)

- Intestinal protozoan infections: diagnosis, prophylaxis, and control (3 hours)
- Vector-borne protozoan infections: diagnosis, prophylaxis, and control (3 hours)
- Zoonotic and emerging protozoan infections (2 hours)
- Laboratory techniques for the diagnosis of major protozoan diseases in companion animals (laboratory practical sessions): coprology, serology, and molecular techniques (4 hours)
- Morphological identification of arthropod vectors of protozoan infections (laboratory activity, 4 hours)

- Diagnostic techniques in veterinary mycology: Suspected fungal infection, sample collection, direct microscopic examination, cultural examination with recognition of macroscopic and microscopic characteristics of key fungi of veterinary interest, serological and molecular techniques (8 hours).
- Prophylactic and control measures for mycoses, with particular regard to potentially zoonotic ones, antifungal susceptibility testing using the EUCAST method, and applications in veterinary practice (4 hours).
- Management of superficial, cutaneous, subcutaneous, and deep mycosis cases in dogs and cats, discussion of the diagnostic approach and the most appropriate control measures (4 hours).

- Review of the functioning of the different compartments of the immune system, essential for proposing the most suitable vaccination protocols (2 hours).
- Types of vaccines available for dogs and cats and their field application, and analysis of all factors that must be considered when proposing the correct vaccination plan for each individual, with particular attention to maternally derived antibodies (MDA) (4 hours).
- Analysis of the most important infectious diseases of dogs and cats and indications for their proper prevention through the application of the most appropriate vaccination protocols in different contexts (domestic environment, breeding kennels/catteries, dog and cat shelters) - presentation of various clinical cases evaluated together with students for proposing the best vaccination approach (6 hours).
- Overview of vaccine adverse reactions and guidelines for their management (4 hours).
- Practical laboratory exercises on the correct execution of blood sampling, analysis and application of various in-clinic diagnostic tests available on the market, analysis and application of various in-clinic tests for the evaluation of vaccine protection (8 hours).
Educational visit to a dog breeding kennel to apply what has been learned in the field for the correct management of the environment and the different types of animals present (puppies, adults, seniors, pregnant bitches) (8 hours).