Biochemistry, Bacteriology, Virology and Veterinary Immunology

A.Y. 2025/2026
11
Max ECTS
112
Overall hours
SSD
BIO/10 VET/05
Language
Italian
Learning objectives
Aims of the module Biochemistry: (1) Knowledge of the molecular design of life, with particular emphasis on the main processes linked to energy production and storage, signal transduction and expression of genetic information, to allow the student to gain the scientific background necessary to understand physiological and pathological processes in animals presented in future courses; (2) Knowledge of the basic theory of colorimetric-based quantitative biological assay and of key topics related to recombinant DNA methodologies;(3) Knowledge of simple bioinformatic tools to allow the student to acquire key up-to-date information from reliable on-line bibliographic and scientific sources; (4) Understanding the contribution of basic and applied research in veterinary science.
Aims of the module Veterinary Bacteriology, Virology and Immunology: (1) To provide a comprehensive overview of the various implications of microbiology (bacteriology and virology) in the fields of biosecurity, animal husbandry, animal welfare, and animal health as a prerequisite to infectious diseases; (2) To describe the functioning of the body's defenses (innate and acquired) against various possible aggressors in different situations (e.g., young, adult, elderly, stressed animals) and to know how to enhance them (e.g., vaccinations); (3) To learn the basics of immunodiagnostics.
Expected learning outcomes
1- Knowledge and understanding. For the Biochemistry module, the student will acquire basic knowledge related to the catabolism and anabolism of the main constituents of animal organisms, including carbohydrates, proteins, lipids, nucleotides, and nucleic acids, and their related metabolic control and coordination mechanisms. The student will also acquire fundamental theoretical and practical notions for performing simple colorimetric assays. For the Veterinary Bacteriology, Virology, and Immunology module, the student will be able to demonstrate to have acquired the knowledge of the basics of microbiology, the characteristics of classical and unconventional microorganisms (viruses, bacteria, prions), and the fundamentals of biosecurity. The student will also be able to demonstrate knowledge of the functioning of the immune system, connecting the different topics covered.
2- Ability to apply knowledge and understanding. For the Biochemistry module, the acquired basic concepts are integrated to understand the complex biochemical processes occurring in animal organisms, with numerous references to physiological and pathological contexts in which these processes are relevant. The concepts provided on colorimetric techniques are applied to the performance of simple colorimetric assays aimed at measuring biologically important metabolites. For the Veterinary Bacteriology, Virology, and Immunology module, the student will have to demonstrate the ability to apply the knowledge acquired on different microorganisms in order to recognize them for beneficial purposes (useful microorganisms) or, conversely, to combat them (pathogenic microorganisms) depending on the situation. The student must also demonstrate having learned the basic principles of biosecurity and knowing how to apply them according to the situation. Finally, the student has to demonstrate the ability to apply the acquired knowledge of both the immune system in various practical contexts (neonatal, young, elderly animals; stress, malnutrition, immunopathological conditions, basic vaccinology) and the fundamentals of immunodiagnostics.
3- Critical thinking and judgment skills. For the Biochemistry module, students' critical thinking skills are fostered both through discussion of quantitative experimental data obtained in the laboratory and by emphasizing the need to properly utilize information obtained online. For the Veterinary Bacteriology, Virology, and Immunology module, the student must demonstrate the ability to critically evaluate the characteristics of microorganisms (viruses and bacteria) in order to set up correct diagnostic methods and prevention tools, serving as an introduction to the future study of infectious diseases. This ability will be verified through various forms of assessment, such as collegial discussion, the execution of laboratory tests, and the drafting of a report focused on a case study derived from the practical activities.
4- Ability to communicate what has been learned. Students' communication skills are developed both during the various modules of the course and during the exam. In particular, the Biochemistry module is complemented by activities involving the use of IT tools for proper evaluation and presentation of the results obtained in practical exercises. Regarding the exam, which consists of open-ended questions, the ability to summarize, complete, and use correct terminology are also factors in the assessment. For the Veterinary Bacteriology, Virology, and Immunology module, the student must be able to describe, using correct scientific terminology in written and eventually oral form, the acquired knowledge of microbiology and immunology: in particular, the composition of the different microorganisms, how they replicate, how they cause disease, how they are fought (immune defenses, physical and chemical antibacterial agents) and how they are prevented (basic notions of biosecurity, hygienic and vaccine prophylaxis). For the final evaluation, in addition to synthesis skills, completeness of exposition, and the use of correct terminology, argumentative coherence and the ability for critical elaboration and interdisciplinary connection will also be taken into account.
5- Ability to pursue independent study throughout one's life. In the Biochemistry module, the acquisition of a solid foundational knowledge of the main chemical components present in organisms and foods, and the biological processes occurring in living organisms, along with the ability to retrieve both bibliographical and theoretical-practical information from reliable sources using the tools described during the course, equips students for ongoing professional and scientific development. Likewise, at the end of the Veterinary Bacteriology, Virology, and Immunology module, the student will be able to autonomously and objectively research, using official and valid sources, to broaden their knowledge on the biology of microbial agents of veterinary interest, the role of the immune system in physiological and pathological conditions, direct and indirect diagnostic techniques, infection prevention tools, and biosecurity principles.
Single course

This course can be attended as a single course.

Course syllabus and organization

Single session

Prerequisites for admission
Knowledge acquired in the courses of the filter semester (Law n. 26 del 14 marzo 2025). No prerequisites are required
Assessment methods and Criteria
The different modules of the integrated course have different examination methods.

BIOCHEMISTRY
The exam consists of a written test including three open-ended questions, lasting two hours. There is always at least one question related to each of the following general topics: Metabolic Biochemistry and General Biochemistry. The specific chapters of the program relating to Metabolic Biochemistry or General Biochemistry are listed in a separate file on the teacher's myARIEL website. The module grade takes into account the accuracy, precision, and completeness of the answers, the ability to summarize, and the use of appropriate terminology.

BACTERIOLOGY AND VIROLOGY
Written exam and possibly an oral exam: 7 multiple-choice questions (each correct answer is worth 2 points) and 4 open-ended questions (each correct answer is worth a maximum of 4 points). The oral exam is optional for students who have passed the written test with a score of at least 18/30. For this module as well, especially concerning the open-ended questions, the grade takes into account the accuracy, precision, and completeness of the answers, the ability to synthesize, and the use of appropriate terminology.

IMMUNOLOGY
Written exam and possibly an oral exam: 12 multiple-choice questions (each correct answer is worth 1 point) and 3 open-ended questions (each correct answer is worth a maximum of 4 points): therefore, the maximum score for the written test is 24/30. The remaining Immunology points (maximum 6) are acquired by completing a laboratory report relating to the analysis of a case study on topics covered during practical sessions in the classroom and laboratory. The minimum score on the written test that entitles the student to have the report analyzed is 18/30. Students who score less than 24/30 on the written test must take the oral exam, while those who score at least 24/30 can choose to take the oral exam to improve their grade (maximum of 3 points). Open-ended questions and the report can be assigned an additional (+) which acts as a bonus for awarding a lode (cum laude) or if necessary to round up a grade.
For this module too, as for the previous ones, the grade takes into account the ability to express oneself, the correct use of scientific language and terminology, the ability to synthesize, and the accuracy and completeness of the answers.

The final exam grade for Veterinary Biochemistry, Bacteriology, Virology, and Immunology is the result of the weighted average of the grades from the 3 modules of the integrated course.

The exams (8 per academic year) take place in the months of January, February, April, June, July, September, October, and November/December, a deadline established by the degree course council.
Biochemistry
Course syllabus
THEORETICAL LECTURES (32 hours)
Properties of biological membranes and membrane transport (2 hours)
Collagen (1 hour)
Myoglobin and hemoglobin (3 hours)
Properties of enzymes, coenzymes, and enzyme regulatory mechanisms (3 hours)
Biochemical mechanisms of digestion and absorption of the main classes of nutritionally relevant molecules (2 hours)
Carbohydrate metabolism: glycolysis, Pentose Phosphate Pathway, gluconeogenesis, glycogen Metabolism, lactose biosynthesis (6 hours)
Metabolism of triglycerides, fatty Acids, and ketone bodies. Overview of cholesterol metabolism (3 hours)
Krebs cycle and oxidative phosphorylation (3 hours)
Lipoproteins, Fatty Acid Binding Proteins (1 hour)
Amino acid metabolism: common pathways of amino acid catabolism, ammonia metabolism, and the urea cycle. Overview of amino acid biosynthesis (4 hours)
Nucleotide metabolism (1 hour)
Biological signaling (1 hour)
Vitamins (1 hour)
Integrating metabolism: muscle, liver, and brain (1 hour)

PRACTICAL LECTURES (16 hours)
Use of databases and software for describing the relationship between protein structure and function, and the structural basis for the Rational Drug Design approach (2 hours) (classroom with a single computer for each student).
Seminar: Molecular basis of oxidative stress (1 h) (class)
Seminar: Anaerobic metabolism, fermentation and rumen metabolism (2 h) (class)
Spectrophotometry applied to the dosage of metabolites and enzymes, with calculations for the preparation of the tests carried out subsequently in the teaching laboratory (2 hours) (classroom)
Colorimetrical determination of glucose, total protein content and alkaline phosphatase (2 hours) (practical laboratory)
Analysis of experimental data obtained in the laboratory using personal computer. Collective d iscussion of the results (2 hours) (class)
Seminar: Principles of Mass Spectrometry Applied to the Analysis of Biological Samples and Food (2 hours)
Seminar: Principles and Applications of Proteomics (1 hour) (classroom)
Use of personal computer for consulting proteomic databases as an example of an information tool on the properties, expression and function of proteins in physiological and pathological contexts, and for the analysis of scientific literature using PubMed (including full text download of non-open-access articles) (2 hours) (classroom with personal computer)

The BIOCHEMISTRY module contributes to the acquisition of DOC 2 and 9 listed in Logbook 2.0.
DOC 2 is acquired through the use of specific on-line tools describing the drug discovery process by the Structure-Based Drug Design approach, through access to biological databases (as detailed in the course syllabus) and through the application of biochemical techniques previously developed for basic research to quantify molecules and metabolites of veterinary biomedical interest. In order to assess the acquisition of the competence each student is required to submit a report of results obtained in the assays performed in laboratory.
Skill 9 is acquired during an exercitation in which the different types of scientific publications (e.g., full-length papers, reviews, etc.) are described and discussed, along with the meaning of open access publication and approaches to optimizing PubMed searches. To assess the acquisition of the competence each student is required to send to the teacher a PDF of a specific article.
Teaching methods
The course is taught through lectures, in-depth seminars on specific topics, and practical exercises consisting of activities carried out in the teaching laboratories and through the use of computers by each individual student.
Teaching Resources
RECOMMENDED TEXTS (one of the following books chosen by the student):
-D.L. Nelson M.M. Cox Introduzione alla biochimica di Lehninger (Zanichelli)
or
-Campbell Farrell Biochimica (Edises)
Power Point presentations used during the lessons, available on the teacher's myAriel website
(https://myariel.unimi.it/course/view.php?id=6091)
Bacteriology and veterinary virology
Course syllabus
LECTURES
- Introduction to the microbial world and a brief history of microbiology (0.5 hours)
- The prokaryotic cell - morphology and functional anatomy (1.5 hours)
- Bacterial replication and metabolism - reproduction and growth on liquid and solid media (1.5 hours)
- Bacterial genetics - genomic material and gene recombination (1 hour)
- Bacteria as pathogenic agents - virulence factors (1.5 hours)
- Classification of bacteria (1.5 hours)
- Methods for observing and studying microorganisms (wet mounts; main staining methods; notes on electron microscopy) (0.5 hours)
- Notes on microbiological diagnostics (Sample collection and transport) (1.5 hours)
- Bacteriological Diagnostics: Preparation and preservation of media; culturing techniques (2 hours in the classroom)
- Bacteriological Diagnostics: Staining and identification techniques (2 hours in the classroom)
- Bacteriological Diagnostics: Serological tests, Antibiogram, and interpretation of results (2 hours in the classroom)
- Characteristics of viruses (1 hour)
- Virus-host relationship (2 hours)
- Genetics of viruses (0.5 hours)
- Virological diagnostics (1 hour)
- Basics of molecular diagnostics applied to microorganism identification: end-point PCR and quantitative PCR (2 hours in the classroom)
- Classification of viruses and prions (1 hour)
- Basic principles of biosecurity, microorganism control systems (physical and chemical agents), and basics of antibacterial and antiviral chemotherapy (1 hour)

The BACTERIOLOGY AND VIROLOGY module, together with the IMMUNOLOGY module, contributes to providing the knowledge necessary for acquiring the DIC outlined in Logbook 2.0 n. 22 and 29.
DOC no. 22: Sample Handling and Diagnostic Testing - For DOC no. 22 (Collecting, preserving and transporting samples, selecting appropriate diagnostic tests, interpreting and understanding the limitations of test results), the module teaches the foundations for the correct collection of various biological samples, their proper transport, and their equally correct preservation to subsequently subject them to the diagnostic tests most suited to different situations (direct diagnosis through the search for the responsible pathogen or indirect diagnosis through the analysis of the immune response stimulated by it). Furthermore, the module provides the first insights for conducting sound reasoning for the interpretation of results and a critical analysis of the limitations of the tests used.
DOC no. 29: Biosecurity and Biosafety Protocols - For DOC no. 29 (Recommending and evaluating biosecurity and biological safety protocols and correctly applying these principles), the module helps the student perform their practical activities aware of the risks and biosecurity rules which, when correctly applied, help to limit or entirely avoid them.
Teaching methods
Lectures (3 ECTS credits)
Teaching Resources
- Poli G., Dall'Ara P., Martino P.A., Rosati S. (2025): Microbiologia e immunologia veterinaria. 4a ed., EDRA, Milano;
- Course slides made available by the teacher on MyAriel
Veterinary immunolgy
Course syllabus
LECTURES
- Introduction to the course, instructor presentation, explanation of the examination methods (1 hour)
- Antigens and Immunogens: structure, characteristics, properties, practical examples (2 hours)
- Non-Specific Immune Defenses: Cells and molecules involved, recognition of PAMPs and DAMPs by TLRs, practical examples (2 hours)
- Specific Immune Defenses divided as follows:
-- Primary and secondary lymphoid organs, B and T lymphocytes (characteristics, markers, BCR and TCR receptors, functions), memory cells (2 hours);
-- Humoral immunity and antibodies (structure, classes, production, mechanisms of action) (2 hours);
-- Cell-mediated immunity and cytotoxicity (MHC, cytokines and cellular interactions, the different working T lymphocytes) (2 hours);
-- Local immunity at surfaces (MALT, immune exclusion and elimination, immunity in various districts such as the gastrointestinal, respiratory, genitourinary tracts, skin, mammary gland, eye) (2 hours)
- Immune Defenses in Veterinary Practice: Natural and artificial passive immunity, neonatal immunology, immunology in the elderly animal, immunity and stress, how pathogens evade immune defenses (3 hours)
- Notes on Immunopathology: Type I, II, III, and IV hypersensitivity and autoimmune diseases (time permitting)

PRACTICAL SESSIONS (PRACTICALS/WORKSHOPS)
- Approach to Vaccination Practice: classification and types of vaccines, vaccine registration, response following vaccination, herd immunity, factors influencing vaccination practice (single group in the classroom) (2 hours)
- Immunodiagnostics: what it is and its purpose, antibody titer, significance, acute serum, convalescent serum, overview of the main immunodiagnostic techniques (precipitation, agglutination, labeled reagent reactions), practical examples (single group in the classroom) (8 hours)
- Dilutions (theory and practice) (groups of max 30 students in the laboratory) (2 hours)
- The ELISA technique (groups of max 30 students in the laboratory) (2 hours)
- Introduction to rapid (in-clinic) tests for formulating a preliminary diagnostic suspicion directly in the clinic (groups of max 30 students in the laboratory) (2 hours)

The IMMUNOLOGY module, together with the BACTERIOLOGY AND VIROLOGY module, contributes to providing the knowledge necessary for acquiring the DIC outlined in Logbook 2.0 n. 22 and 29.
DOC no. 22: Sample Handling and Diagnostic Testing - For DOC no. 22 (Collecting, preserving and transporting samples, selecting appropriate diagnostic tests, interpreting and understanding the limitations of test results), the module teaches the foundations for the correct collection of various biological samples, their proper transport, and their equally correct preservation to subsequently subject them to the diagnostic tests most suited to different situations (direct diagnosis through the search for the responsible pathogen or indirect diagnosis through the analysis of the immune response stimulated by it). Furthermore, the module provides the first insights for conducting sound reasoning for the interpretation of results and a critical analysis of the limitations of the tests used.
DOC no. 29: Biosecurity and Biosafety Protocols - For DOC no. 29 (Recommending and evaluating biosecurity and biological safety protocols and correctly applying these principles), the module helps the student perform their practical activities aware of the risks and biosecurity rules which, when correctly applied, help to limit or entirely avoid them.
Teaching methods
Lectures (2 ECTS credits), seminars, and laboratory practicals (1 ECTS credit).
Teaching Resources
- Poli G., Dall'Ara P., Martino P.A., Rosati S. (2025): Microbiologia e immunologia veterinaria. 4a ed., EDRA, Milano;
- Course slides made available by the teacher on MyAriel (https://myariel.unimi.it/course/view.php?id=7070)
Modules or teaching units
Bacteriology and veterinary virology
VET/05 - INFECTIOUS DISEASES OF DOMESTIC ANIMALS - University credits: 3
Practicals: 16 hours
Lessons: 16 hours
Professor: Riva Federica
Shifts:
1- Turno unico per tutti gli studenti
Professor: Riva Federica
2 - 4 turni da 6 ore per un gruppo di studenti
Professor: Riva Federica
Turno
Professor: Riva Federica

Biochemistry
BIO/10 - BIOCHEMISTRY - University credits: 5
Practicals: 16 hours
Lessons: 32 hours
Professor: Negri Armando
Shifts:
1- turno unico per tutti gli studenti
Professor: Negri Armando
2- 4 turni da 2 ore per gruppi di studenti
Professor: Negri Armando
Turno
Professor: Negri Armando

Veterinary immunolgy
VET/05 - INFECTIOUS DISEASES OF DOMESTIC ANIMALS - University credits: 3
Practicals: 16 hours
Lessons: 16 hours
Shifts:
1- Turno unico per tutti gli studenti
Professor: Dall'Ara Paola Emanuela Maria
2 - 4 turni da 6 ore per un gruppo di studenti
Professor: Dall'Ara Paola Emanuela Maria

Professor(s)
Reception:
contact me by mail or phone