This course aims mainly to provide theoretical bases for improvement and management of biotechnological processes and the use of enzymes technology for production of compounds by industrial bioprocesses. The course is ideally linked to those dealing with protein engineering, structural biology, enzymology, bioinformatics.
Risultati apprendimento attesi
At the end of this class, the students are expected to: 1) have acquired understanding of importance of metabolic pathways on process development; 2) have understood the rational of metabolic engineering strategies (approaches and methods) utilized to improve cellular performance; 3) have acquired understanding of theoretical aspects and experimental approaches to engineer microorganisms in the context of biotechnological applications; 4) be able to recognize some relevant applications of free or immobilized enzymes for the biotransformations of both natural and non-natural substrates in terms of enzymes involved, products obtained and operational details. 5) have acquired the ability to correlate the disciplines involved and their relevance to biotechnological developments.
Periodo: Primo semestre
(se vi sono più edizioni controllare anche il periodo dell'edizione, che può essere diverso)
Prof. Compagno and Prof. Morelli will share teaching of this class by focusing on metabolic engineering strategies utilized to improve cellular metabolism and the use of enzymes technology to improve industrial production processes, respectively. The lectures will first review information on industrial microbiology, cultivation strategies (batch, fed-batch, continuous culture) the principles of enzyme catalysis and biotechnological application. Lectures will cover: tools to study metabolic flux balance and to engineering cells; analysis of regulation of carbon source metabolism and strategies for utilizing alternative carbon sources; strategies to redirect metabolic pathways for improving production and to introduce new pathways for production of new compounds; strategies to increase the cellular performance to improve industrial processes; selected applications for the production of drug intermediates, natural and unnatural amino acids through hydrolase-catalyzed kinetic resolution; biotechnological applications of enzymes (lipase, amylase, pectinolytic enzymes dehydrogenases, aldolases).
A revision of the topics covered by the basic biochemistry and molecular biology classes included in the bachelor curriculum before attending the course is highly recommended.
Teaching Mode: Classroom lectures supported by projected material with discussions on experimental design, data analysis, and specific case studies. Attendance is highly recommended.
Materiale di riferimento
Copies of the slides projected in the classroom as well as other materials will be made available at the beginning of the course. By no means this material replaces the lectures. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
Modalità di verifica dell’apprendimento e criteri di valutazione
Compagno part: the evaluation of the student's performance will be based on a written examination with open-answer questions spanning all topics covered in the class. This mode will offer the opportunity to test by writing students' ability to apply their knowledge on topics covered by this class. Morelli part: written examination, consisting of two parts. Part 1: two multiple-choice questions, aimed at verifying (i) the ability of the students in recognizing enzyme-catalyzed chemical transformations and (ii) the knowledge of relevant properties of enzymes used as biocatalysts. Part 2: open-answer question about applications of biocatalysts. Questions will be focused and limited but will require synthesis and connection skills, as they might span different topics covered during the course. The final grade will result from the joint evaluation of each candidate by the two teachers.