Biotecnologie: modelli sperimentali nella ricerca

A.A. 2019/2020
12
Crediti massimi
102
Ore totali
SSD
AGR/18 AGR/20 VET/07
Lingua
Inglese
Obiettivi formativi
The aim of the course is to present basic and advanced methodologies in the field of biotechnological research. Several class activities will give students useful tools to develop a critical ability in identifying and choosing experimental models in relation to their applicability.
Risultati apprendimento attesi
At the end of the course, the student will know:  the main basic and advanced methodologies in the research field and will acquired the skills allowing him to identify the most suitable experimental models for biotechnological research. Specifically, students will acquire knowledge on:
cell culture techniques
3Rs in toxicology and Replacement
alternative methods in toxicology and application
lab animals wellbeing, management and rearing techniques  
Programma e organizzazione didattica

Edizione unica

Responsabile
Periodo
Secondo semestre
Prerequisiti
Basic knowledge in toxicology is required.
Modalità di verifica dell’apprendimento e criteri di valutazione
The exam consists of a written evaluation aimed to evaluate knowledge and understanding of the lectures' subjects and of the laboratory activities. The test will be evaluated in thirtieths (minimum grade is 18 and the maximum is 30 "cum laude", with honors). The final score of course is the sum of the scores of the three teaching units. The score will take into account the accuracy and the capacity to motivate the answers, as well as the capacity to explain the concepts acquired with appropriate and updated terminology.
Mod. 1: Colture cellulari
Programma
Lectures
Design and equipment for a cell culture laboratory
Cell Culture - basic principles
- Main types of cell culture: primary cultures and cell lines
- The cell environment: sterile environment, pH, temperature and supply of nutrients for growth
- Basic techniques for cell culture: suspension culture, monolayer culture, 3D culture
- Advanced technique for cell culture: insert, scaffold and mammosphere
- Quality control
- Protocols for cell quantification and viability
Cell culture protocols and application to the field of animal nutrition
- Cell culture protocols for research: differentiation, tissue remodelling and nutrigenomic
- Epithelial cell barrier: trans epithelia transfer of nutrients, feed bioactive compound activity
- Cell culture for evaluation of feed functional and health claims

Journal club
Critically evaluation of recent articles in scientific literature (work group)

Exercises:
- The "Do's and Don'ts" of cell culture: aseptic technique and good cell culture practice
- Preparation and storage of culture media
- Cryopreservation and storage of cell culture
- Cytotoxicity and viability assay
- Culture of cell lines
- Cell-based assays for in vitro testing of functional properties of feed bioactive compounds and additives: probiotic adhesion tests
- National cell culture laboratories and cell bank: visit
Metodi didattici
Lectures and laboratory activity
Materiale di riferimento
Didactic material available on ARIEL: https://fchelicc.ariel.ctu.unimi.it/v5/home/Default.aspx
Culture of Animal Cells: A Manual of Basic Technique. R. Ian Freshney, Wiley-Liss, 2000.
Animal Cell Culture: A Practical Approach. John R. W. Masters, Oxford University Press, 2000.
Animal Cell Culture and Technology. Mike Butler, Michael Butler, Taylor & Francis, Inc., 2004.
Introduzione alle colture cellulari, Mariotti GL et al., Tecniche Nuove, 2010
Mod. 2: Metodi alternativi in tossicologia
Programma
Lectures
Toxicology, Alternative Methods and 3Rs Replace, Refine, Reduce (2 hours)
Alternative Methods and Validation (2 hours)
Replacement: validated in vitro test (4 hours)
Integrating Testing Strategy (1 hours)
Skin sensitization (2 hours)
Endocrine disruptor and alternative methods (1 hour)
Carcinogenesis (2 hours)
Reproductive Toxicology and Alternative methods (2 hours)
Botulin Toxin and 3Rs (1 hour)
Laboratory activity
In vitro markers in toxicity testing (2 hours)
In silico methods (4 hours)
ITS, Integrating Testing Strategy examples (2 hours)
Educational visit in Italian laboratory (4 hours)
Metodi didattici
Lectures and laboratory activity
Materiale di riferimento
Mod. 3: Benessere dell'animale da laboratorio "Animal care"
Programma
Lectures
Management and breeding of transgenic colonies of Rodents (2 hours)
Management and breeding of Zebrafih (Danio rerio) (2 hours)
Anesthesia, analgesia and euthanasia: D.L. 4 marzo 2014 n. 26 (2 hours)
Dosing and sampling (2 hours)
The experimental protocol in biomedical research (2 hours)
Adoptions (1 hour)
Nematodes in research (1 hour)
Tutorials
Non-invasive imaging techniques: Thermography (2 hours)
Murine models (3 hours)
Visit a research facility (4 hours)
Visits to breeding of laboratory animals (3 hours)
Metodi didattici
Lectures and tutorial activity
Materiale di riferimento
Didactic material available at www.fabioluzi.it
Moduli o unità didattiche
Mod. 1: Colture cellulari
AGR/18 - NUTRIZIONE E ALIMENTAZIONE ANIMALE - CFU: 5
Esercitazioni: 36 ore
Lezioni: 12 ore

Mod. 2: Metodi alternativi in tossicologia
VET/07 - FARMACOLOGIA E TOSSICOLOGIA VETERINARIA - CFU: 4
Esercitazioni: 12 ore
Lezioni: 18 ore

Mod. 3: Benessere dell'animale da laboratorio "Animal care"
AGR/20 - ZOOCOLTURE - CFU: 3
Esercitazioni: 12 ore
Lezioni: 12 ore
Docente: Luzi Fabio

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Via Celoria 10 - Milano
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Sede di Via Trentacoste, 2
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su appuntamento telefonando al 0250318053 o scrivendo a: fabio.luzi@unimi.it
Studio del Docente - Dipartimento DIMEVET - Via Celoria, 10 Milano