From 3d-culture and 3d-printing to organoids
A.A. 2020/2021
Obiettivi formativi
Cells in vivo are in a three-dimensional environment. Cells cultured in 3D models have biophysical characteristic and biomechanical signals, which more accurately simulate normal cell morphology, proliferation, differentiation and migrations. The objective of the course is to give a comprehensive review of the main aspects and methods for 3D cell models from 3D-culture and 3d-printing to organoids.
Risultati apprendimento attesi
At the end of the course, the student acquires a solid knowledge of in vivo cell environment. This knowledge finds applications to the use of appropriate 3D culture systems with biophysical and biomechanical features that more accurately simulate normal cell morphology, proliferation, differentiation and migrations.
Periodo: Secondo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Secondo semestre
Prerequisiti
None
Modalità di verifica dell’apprendimento e criteri di valutazione
Oral and practical test
Modulo: Cell interaction and organoids
Programma
Mechano-sensing
Mechanotransduction
Shearing
Stiffness materials and cell responses
How to select the adequate surface
Citoskeleton and ECM
Macro-crowding
Mechanotransduction
Shearing
Stiffness materials and cell responses
How to select the adequate surface
Citoskeleton and ECM
Macro-crowding
Metodi didattici
Classical lectures,practical classes, webinars
Materiale di riferimento
Selected review articles, web-sites and webinars
Modulo: 3 D approach to cell culture
Programma
3D-culture types
Methods and protocols for 3D-cultures
Critical factors to consider for each 3D-model system and cell type
Examples of applications of 3D-culture models
Practicals will be dedicated to 3D-culture
Methods and protocols for 3D-cultures
Critical factors to consider for each 3D-model system and cell type
Examples of applications of 3D-culture models
Practicals will be dedicated to 3D-culture
Metodi didattici
Classical lectures, practical classes, webinars
Materiale di riferimento
3D Cell Culture Methods and Protocols. Editors: Haycock, John (Ed.). Humana press. 2011.
Selected review articles, web-sites and webinars
Selected review articles, web-sites and webinars
Modulo: 3 D bioprinting and practicals
Programma
3D-bioprinters: philosophy and application in medicine
Artificial and biological matrix
Fabrication process
Decellularization & Recellularization process
"Print your heart out 3D". How to design, create and implant and ad hoc organoid
Artificial and biological matrix
Fabrication process
Decellularization & Recellularization process
"Print your heart out 3D". How to design, create and implant and ad hoc organoid
Metodi didattici
Classical lectures,practical classes, webinars
Materiale di riferimento
Selected review articles, web-sites and webinars
Moduli o unità didattiche
Modulo: 3 D approach to cell culture
AGR/18 - NUTRIZIONE E ALIMENTAZIONE ANIMALE - CFU: 3
Lezioni: 18 ore
Docente:
Cheli Federica
Modulo: 3 D bioprinting and practicals
VET/09 - CLINICA CHIRURGICA VETERINARIA - CFU: 2
Lezioni: 12 ore
Docente:
Acocella Fabio
Modulo: Cell interaction and organoids
VET/01 - ANATOMIA DEGLI ANIMALI DOMESTICI - CFU: 3
Lezioni: 18 ore
Docente:
Gandolfi Fulvio
Docente/i
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