From 3d-culture and 3d-printing to organoids

A.Y. 2020/2021
8
Max ECTS
48
Overall hours
SSD
AGR/18 VET/01 VET/09
Language
English
Learning objectives
Cells in vivo are in a three-dimensional environment. Cells cultured in 3D models have biophysical characteristic and biomechanical signals, which more accurately simulate normal cell morphology, proliferation, differentiation and migrations. The objective of the course is to give a comprehensive review of the main aspects and methods for 3D cell models from 3D-culture and 3d-printing to organoids.
Expected learning outcomes
At the end of the course, the student acquires a solid knowledge of in vivo cell environment. This knowledge finds applications to the use of appropriate 3D culture systems with biophysical and biomechanical features that more accurately simulate normal cell morphology, proliferation, differentiation and migrations. The student also acquires the ability to print very simple scaffolds using a 3D printer and to select the adequate matrix, encapsulate cells/tissues in microbioreactors and perform basic organ decellularization protocols
Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
Prerequisites for admission
None
Assessment methods and Criteria
Oral and practical test
Modulo: Cell interaction and organoids
Course syllabus
Mechano-sensing
Mechanotransduction
Shearing
Stiffness materials and cell responses
How to select the adequate surface
Citoskeleton and ECM
Macro-crowding
Teaching methods
Classical lectures,practical classes, webinars
Teaching Resources
Selected review articles, web-sites and webinars
Modulo: 3 D approach to cell culture
Course syllabus
3D-culture types
Methods and protocols for 3D-cultures
Critical factors to consider for each 3D-model system and cell type
Examples of applications of 3D-culture models
Practicals will be dedicated to 3D-culture
Teaching methods
Classical lectures, practical classes, webinars
Teaching Resources
3D Cell Culture Methods and Protocols. Editors: Haycock, John (Ed.). Humana press. 2011.
Selected review articles, web-sites and webinars
Modulo: 3 D bioprinting and practicals
Course syllabus
3D-bioprinters: philosophy and application in medicine
Artificial and biological matrix
Fabrication process
Decellularization & Recellularization process
"Print your heart out 3D". How to design, create and implant and ad hoc organoid
Teaching methods
Classical lectures,practical classes, webinars
Teaching Resources
Selected review articles, web-sites and webinars
Modulo: 3 D approach to cell culture
AGR/18 - ANIMAL NUTRITION AND FEEDING - University credits: 3
Lessons: 18 hours
Professor: Cheli Federica
Modulo: 3 D bioprinting and practicals
VET/09 - VETERINARY CLINICAL SURGERY - University credits: 2
Lessons: 12 hours
Professor: Acocella Fabio
Modulo: Cell interaction and organoids
VET/01 - VETERINARY ANATOMY - University credits: 3
Lessons: 18 hours
Professor: Gandolfi Fulvio
Professor(s)
Reception:
From Monday to Friday upon appointment
Office or after the lectures