Fermentation biotechnology

A.A. 2020/2021
Crediti massimi
Ore totali
Obiettivi formativi
This course aims to provide the theoretical bases of the strategies employed to set-up, manage and improve microbial biotechnological processes, for the industrial production of compounds. Focus is on bioreactor technology, scale-up strategies, understanding the importance of metabolic pathways on process development and on metabolic engineering strategies utilized to engineer microorganisms in the context of biotechnological applications. Several industrial examples will be showed and critically discussed.
Risultati apprendimento attesi
Students will develop the capabilities for setting-up and manage an industrial fermentation process using microbial cells. More in detail they will acquire specific tools (bioreactor set-up and fermentation control, strain improvement, metabolic flux analysis and engineering, statistical analyses) in order to meet the demand of high productive and efficient industrial bioprocesses.
Programma e organizzazione didattica

Single session

Primo semestre
Lessons on the" industrial bioprocesses development" module will be carried out live on MS teams; slides of the lessons, together with additional materials will be available on Ariel website.
Lessons on the "improvement of bioprocesses in yeast" module will be carried out by Power point with audio made by the teacher and will be available on Ariel website, uploaded at the time scheduled for the lessons.
Three meetings will be arranged in class.

The examination consists in two interviews.
The interview on the "industrial bioprocesses development" module will be by MS teams
The interview on the "improvement of bioprocesses in yeast" module will be by Skype call.
The final score will be a weighted mean based on the credits of the two modules of the course.
The course is organised in two units: Unit 1 -Industrial Bioprocess Development (4CFU, Diego Romano); Unit 2 - Improvement of bioprocesses in yeast (3CFU, Concetta Compagno). The specific program of each unit is reported in the following sections.

Unit 1: Industrial Bioprocesses Development, - D. Romano (4 CFU)
Introduction to bioprocesses: bioreactor design, fermentation techniques (batch, fed batch, continuous), scale-up and scale down approaches, downstream processing, experimental design applied to industrial fermentations. Industrial examples: production of aminoacids (glutamic acid, lysine), organic acids (citric, lactic), solvents, biofuels (ethanol, butanol), flavours (vanilline), antibiotics; production of recombinant proteins in prokaryotes. New generation of bioprocesses: biomaterials (polyamide, polylactic-acid, bacterial cellulose, polyhydroxyalkanoates), fermentation of gaseous substrates (CO2, CH4).
Unit 2: Improvement of bioprocesses in yeast, - C. Compagno (3 CFU)
Strategies to improve the metabolic performance of yeast cells and production process:
metabolic flux balance analysis;
recombinant technology in yeast,
regulation of carbon source metabolism in yeasts.
Strategies to redirect existent metabolic pathways improving industrial processes.
Strategies to utilize alternative carbon sources for biofuels production.
Strategies to increase stress tolerance for industrial processes.
Strategies to introduce new pathways for production of new compounds.
Students must know fundamentals of microbial physiology (biosynthetic pathways of primary metabolism, microbial life cycles), enzymatic catalysis and molecular biology, organic chemistry (main reactions of carboxylic acids, ketones, aldehydes, amines).
Metodi didattici
Unit 1 -Industrial Bioprocess Development - Frontal lessons: 4CFU
Unit 2 - Improvement of bioprocesses in yeast - Frontal lessons - 3CFU
Materiale di riferimento
Slides of the lessons and the list of scientific articles will be available during the course and will be available for students on the "Ariel" platform.
Books: Industrial Biotechnology-Products and Processes. 2017 Wiley-VCH Verlag GmbH & Co. Print ISBN: 978-3-527-34181-8
Modalità di verifica dell’apprendimento e criteri di valutazione
The examination consists in an interview about the "industrial bioprocesses development" module, and a written test on the "improvement of bioprocesses in yeast" module. The score will be a weighted mean based on the credits of the two modules of the course. Course prerequisites and exam arrangements are the same for students attending and not attending the course lessons. Lesson attendance is nevertheless strongly encouraged.
Moduli o unità didattiche
Improvement of bioprocesses in yeast
Lezioni: 24 ore

Industrial Bioprocesses Development
Lezioni: 32 ore
Docente: Romano Diego

lunedì 14.30-16.30
via Mangiagalli 25, terzo piano