The course's aim is to allow students to acquire knowledge about fermentation technologies and industrial production of biotechnological relevant products. In particular, the subjects will concern fermentation systems associated with the use of microorganisms based on their specific metabolic pathways involved in product synthesis. The course include also a part of laboratory practice in order to acquire some technical skills on fermentations and analytical tools.
Expected learning outcomes
At the end of this class, the students are expected to have acquired knowledges about the fermentation systems and their biotechnological importance, the development and the improvement of these processes, the importance of microorganisms and their metabolic pathways on process development. By attending the laboratory the students will acquire competences about how to perform a fermentation process and how to analyse some products.
Lesson period: Second semester
(In case of multiple editions, please check the period, as it may vary)
Industrial microorganisms: methodologies for strains improvement and storage. Cultivation technology Bioreactors design Substrates for industrial productions Batch, continuous culture and fed-batch Immobilization of cells and enzymes Monitoring of bioprocess: analysis of bio-parameters Biosynthesis of ethanol Biosynthesis of lactic acid Biosynthesis of acetone/butanol Biosynthesis of amino acids (L-glutamic acid, L-lysine) Biosynthesis of antibiotics Practice in laboratory Analysis of batch culture: glucose/ethanol assay Analysis of bioconversion process Assay of industrial enzymes
Prerequisites for admission
A good knowledge on Microbiology and Biochemistry is required.
Classroom lectures and laboratory pratic.
The slides used will be available at the biginning of the course. Suggested books: Biotecnologie Microbiche, Casa Editrice Ambrosiana Biotecnologie di base, Zanichelli
Assessment methods and Criteria
The evaluation of the student's performance will be based on a written examination with open-answer questions spanning topics covered in the class. This mode will offer the opportunity to test by writing students' ability to apply their knowledge on topics covered by this class.