The main objective of the course is to provide the student with the basic knowledge in imaging techniques with optical, electronic, probe scanning, diffusion-MRI and functional-MRI imaging with reference to applications in the biomedical field. Visits will be made to laboratories equipped with the instruments described in the course. The course also includes a series of seminars of experts in the machine learning and lab-on-chip devices.
Expected learning outcomes
At the end of the Course, the student will learn the fundamental elements of imaging techniques with applications in particular to the biomedical field. The student will acquire the ability to evaluate the most suitable technique / methodology for structural and functional imaging of biological samples at the level of molecular, cellular, and tissue structures up to complex organs / organisms.
Lesson period: First semester
(In case of multiple editions, please check the period, as it may vary)
I part Bioimaging techniques with fluorescent probes: confocal and two-photon microscopy, STED, FRET, FRAP, TIRF. SEM, TEM, HRTEM and cryo-TEM electron microscopy. Scanning microscopy of AFM probe. Lab-on-chip and Cell-on-chip systems for biomedical applications.
II part History of magnetism in medicine. Recalls of MRI principles. Images reconstruction. Functional MRI. Diffusion-MRI. Nanomagnetism and magnetic techniques. MRI contrast agents. Magnetic transport (in biological systems). Magnetic hyperthermia in medicine. Hyperthermic agents. systems for biomedical applications.
Prerequisites for admission
Basic knowledge of Structure of Matter and Quantum Mechanics.
Lessons with slides in PowerPoint.
All the slides of the lessons are on Ariel.
I parte * A. Diaspro et al. - Nanoscopy and Multidimensional Optical Fluorescence Microscopy. ed. Chapman and Hall/CRC * J.J. Bozzola et L. D. Russel - Electron Microscopy. Jones & Bartlett Learning
II parte * Haacke - Magnetic Resonance Imaging - Physical Principles and Sequence Design. ed J.Wiley * Varadan et al., Nanomedicine Design And Applications Of Magnetic Nanomaterials Nanosensors And Nanosystems. ed. Wiley
Assessment methods and Criteria
Presentation of a scientific article and oral exam on the topics covered in the course.