Microbiological Techniques
A.Y. 2019/2020
Learning objectives
Main purpose: to supply the cultural and operative instruments necessary for relating the acquired knowledge in the microbiological field to the microbiological methods and their application in the field of biotechnology.
Expected learning outcomes
Planning, organization and carriyng out of experimental analyses for the phenotypic and genotypic chracterization of microorganisms involved in the food industry.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Lesson period
Second semester
Course syllabus
Lectures:
Isolation, selection and survey of microorganisms from heterogeneous matrices. Taxonomic identification of bacteria: phenotypic and genotypic characteristics. Planning and choise of experimental analyses for phenotypic and genotypic identification. Genetic organization in bacteria. The measurable characteristics of plasmidic and chromosomal DNA. The concepts of molecular genetics and their application in both basic and applied biotechnology (with particular regard to techniques that allow precise detection and amplification of DNA through the polymerase chain reaction or PCR)
Laboratory exercises:
Culture media preparation. Use of autoclaves and filters for the sterilization of media and materials. Isolation techniques. Measurement of bacterial growth. Obtainment of pure cultures. Planning and carrying out of phenotypic analyses for bacterial identification and characterization. Extraction of chromosomal and plasmidic DNA and their study: separation and visualization through gel electrophoresis techniques. PCR experiments
Isolation, selection and survey of microorganisms from heterogeneous matrices. Taxonomic identification of bacteria: phenotypic and genotypic characteristics. Planning and choise of experimental analyses for phenotypic and genotypic identification. Genetic organization in bacteria. The measurable characteristics of plasmidic and chromosomal DNA. The concepts of molecular genetics and their application in both basic and applied biotechnology (with particular regard to techniques that allow precise detection and amplification of DNA through the polymerase chain reaction or PCR)
Laboratory exercises:
Culture media preparation. Use of autoclaves and filters for the sterilization of media and materials. Isolation techniques. Measurement of bacterial growth. Obtainment of pure cultures. Planning and carrying out of phenotypic analyses for bacterial identification and characterization. Extraction of chromosomal and plasmidic DNA and their study: separation and visualization through gel electrophoresis techniques. PCR experiments
Prerequisites for admission
The student must have already attended courses on microbiological disciplines
Teaching methods
The teacher uses lectures and individual laboratory training
Teaching Resources
Slides and books are available on Ariel web platform. The professor is available for questions and further clarifications by appointment
Assessment methods and Criteria
The exam is organized in an oral test. The evaluation parameters include the assessment of the skills acquired, the capacity for critical reasoning, the quality of exposure and the use of specialized vocabulary.
AGR/16 - AGRICULTURAL MICROBIOLOGY - University credits: 4
Laboratories: 16 hours
Lessons: 24 hours
Lessons: 24 hours
Professor:
Fortina Maria Grazia
Shifts:
-
Professor:
Fortina Maria Grazia