Biotechnology applied to animal health and production

A.Y. 2021/2022
Overall hours
Learning objectives
The course focus on the relationship between applied biotechnology and animal welfare in farm, laboratory and companion animals. Oxidatively generated stress, related causes and the relationship between oxidative status and farming systems are discussed. Biomarkers of oxidative stress applied to the evaluation of animal health status are considered.
Expected learning outcomes
At the end of the course, students will have knowledge on the main basic and advanced biotechnological methodologies applied to livestock and companion animals, and will acquired skills to use some bioinformatics tools and software. Moreover, acquired knowledges will concern the identification and application of specific biomarkers for oxidative stress evaluation related with animal welfare.
Course syllabus and organization

Single session

Lesson period
First semester
More specific information on the delivery modes of training activities for a.y. 2021-22 will be provided over the coming months, based on the evolution of the public health situation.
Course syllabus
Livestock production in Europe and Italy: recent biotechnology trends and future prospects.
Characteristics of the available markers and their possible applications in the diagnosis of hereditary pathologies, paternity and maternity test and in genomic selection.
Elements of Bioinformatics: theoretical and practical approach to bioinformatics tools and to the online genetic and bibliographic databases.
Lab work
Mitochondrial DNA and genomic DNA sequence analysis using dedicated softwares and interpretation of the results.
Access to on-line databases: how to use bioinformatic tools to interpret results of genomic data.
Practical approach to the primer design rules by specific tools. Tools for the analysis with restriction enzymes.

Markers of oxidative stress.
Occurrence of radical species.
Causes and occurrence of oxidative stress damage.
Evaluation of total anti-oxidant capacity and repairing mechanisms.
Antioxidant agents in biological systems and activity.
Identification of valid and reliable biomarkers in monitoring oxidative status in biological systems.
Prerequisites for admission
Prerequisites are not required.
Teaching methods
Lectures and practical in the computer lab.
Teaching Resources
Class notes made available from the lecturer on ARIEL database. Notices concerning any update related to the evolution of Covid-19 will be published on the Ariel website.
Assessment methods and Criteria
The exam consists of an oral interview aimed to test knowledge, competences and the correct understanding of contents of the course.
Oral exams, if not in presence, will be carried out using the MTeams platform.
Unita' didattica: Markers di stress ossidativo nel benessere animale
AGR/19 - ANIMAL SCIENCE - University credits: 2
Lessons: 12 hours
Unita' didattica: Metodologie molecolari applicate alle produzioni animali
AGR/19 - ANIMAL SCIENCE - University credits: 3
Practicals: 12 hours
Lessons: 12 hours