Applied Microbiology and Hygiene
A.Y. 2022/2023
Learning objectives
In this course, students will acquire basic knowledge of microbiology. The students will learn about the structure and the function of the prokaryotic cell and will familiarize with the metabolic and physiological diversity of prokaryotes. Key concepts on genetics will be also offered to students. Overall these concepts represent tools to understand the applications of microbiology in the medical, pharmaceutical and diagnostic fields as well as in food industry.
The course will also introduce the students to the fundamental mechanisms of bacterial pathogenesis, the main infectious diseases and host immune defenses. Such knowledge is relevant for an effective management and control of infectious diseases.
The course will also introduce the students to the fundamental mechanisms of bacterial pathogenesis, the main infectious diseases and host immune defenses. Such knowledge is relevant for an effective management and control of infectious diseases.
Expected learning outcomes
On successful completion of this course, the student will have acquired basic knowledge in microbiology and will know in details the functioning of the prokaryotic cell. Moreover, the student will become familiar with the most important infectious diseases and the strategies for their control.
Lesson period: Activity scheduled over several sessions (see Course syllabus and organization section for more detailed information).
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Linea AL
Responsible
Lesson period
year
More specific information on the delivery modes for the academic year 2022/2023 will be provided over the coming months based on the evolution of the public health situation. Lessons will follow the canonical lessons timetable published on the website. Teaching material will be deposited in the ARIEL platform. Students willing to follow face to face lessons will have to reserve their place using the dedicated App
The exam is written and consists of multiple choice and/or open questions. Maximal score related to complexity of questions. Final score is the sum of scores obtained in the different sections of the exam. The exam, whenever possible, will be held face to face. In case face to face mode will not be possible because of the covid outbreak the exam will be written and held using the Microspft teams platform.
The exam will evaluate
- The ability of the student to reach course objectives in terms of knowledge and understanding
- The ability of the student to apply knowledge and understanding
- The ability of the student to use the correct terminology in the field of Microbiology and to discuss the different topics in a clear and rational way
The exam is written and consists of multiple choice and/or open questions. Maximal score related to complexity of questions. Final score is the sum of scores obtained in the different sections of the exam. The exam, whenever possible, will be held face to face. In case face to face mode will not be possible because of the covid outbreak the exam will be written and held using the Microspft teams platform.
The exam will evaluate
- The ability of the student to reach course objectives in terms of knowledge and understanding
- The ability of the student to apply knowledge and understanding
- The ability of the student to use the correct terminology in the field of Microbiology and to discuss the different topics in a clear and rational way
Course syllabus
TEACHING UNIT OF APPLIED MICROBIOLOGY (4 CFU - 32 hours)
- Overview of microbiology and its applications.
- Overview of cell structure: prokaryotes and eukaryotes. Bacteria and Archaea. Cytoplasmic membrane, structure and function. Bacterial transport systems. Cell wall of prokaryotes. Inhibitors of cell wall biogenesis. Outer membrane of Gram-negative bacteria. Capsules. Flagella and bacterial motility. Surface structure for adhesion: pili. The nucleoid. Structure and organization of bacterial chromosome. Inhibitors of DNA replication. Transcription and inhibitors. Cytoplasm. Ribosomes and inhibitors of translation. Cell inclusions. Endospores.
- Microbial growth and microbial growth control. Principles of microbial nutrition. Liquid and solid media, growth conditions. Methods for sterilization. Disinfectants and antiseptics.
- Nutrition and metabolism. Overview of metabolism. Fermentation and substrate level phosphorylation. Aerobic and anaerobic respiration. Chemiolithotrophy.
- Microbial genetics. Mutations and mutants. Horizontal gene transfer mechanisms: Transformation, Conjugation Transduction.
- Antibiotics. Structure and mechanisms of action. Minimal inhibitory concentration (MIC). Overview of resistance mechanisms.
TEACHING UNI: VIROLOGY (2 CFU - 16 ore)
- Introduction to Virology: what is a virus? Structure and classification of viruses. Laboratory methods to study the infectious cycle. Biocontainment laboratories (BSL1 to BSL4).
- Viral replication cycles. Attachment and entry. Gene expression and genome replication. The Baltimore classification. Assembly and egress.
- Virus-host interaction. Mechanisms of pathogenesis. Viral oncogenesis.
- Viral diseases and antiviral drugs. AIDS and retroviral drugs. Herpes viruses and anti-herpetic drugs. Viral hepatitis and antivirals targeting HBV and HCV. Respiratory viral infections: influenza and Covid-19.
TEACHING UNIT: HYGIENE (2 CFU - 16 hours)
- Introduction to Hygiene. Health and diseases. Public health. Demographic studies.
- Descriptive epidemiology: measures of frequency, ratios, proportions, crude and adjusted rates. Disease prevalence and incidence.
- Anaytic and experimental epidemiology. Causality criteria. Cross-sectional vs longitudinal studies. Cohort and case-control studies. Relative risk and odds ratio. Clinical trials and preventive interventions.
- Preventive medicine and promotion of health. Primary, secondary and tertiary prevention. Mass screening campaigns. Sensitivity and specificity of screening tests. Oncological screening programs in Italy.
- General epidemiology of infectious diseases. The epidemiological transition. Koch's postulates. The chain of infection. Sources and reservoirs of infection. Modes of transmission. Patterns of infectious disease occurence: endemic, epidemic and pandemic diseases.
- Prophylaxis measures in infectious disease. Direct and indirect prophylaxis: notification, quarantine measures, diagnostic ascertainment, health surveillance, epidemiologic survey, disinfection, disinfestation, sterilization.
- The immune response: anatomical and physical barriers. Innate and adaptive immune responses. Humoral and cellular immune response. The role of B and T lymphocytes and antibodies.
- History of Vaccines. Vaccine-preventable diseases. Vaccine types: life-attenuated vaccines; inactivated vaccines; toxoid vaccines; subunit , recombinant, polysaccharide, and conjugate vaccines. Adjuvants. Administration modes, side effects and contraindications. National vaccine plan. Passive immunization and immune-prophylaxis.
- Epidemiology and prophylaxis of transmittable disease: airborne infectious diseases; sexually-transmitted diseases; chronic viral hepatitis; diseases transmitted via oral-fecal route; vector-borne infectious diseases.
- Overview of microbiology and its applications.
- Overview of cell structure: prokaryotes and eukaryotes. Bacteria and Archaea. Cytoplasmic membrane, structure and function. Bacterial transport systems. Cell wall of prokaryotes. Inhibitors of cell wall biogenesis. Outer membrane of Gram-negative bacteria. Capsules. Flagella and bacterial motility. Surface structure for adhesion: pili. The nucleoid. Structure and organization of bacterial chromosome. Inhibitors of DNA replication. Transcription and inhibitors. Cytoplasm. Ribosomes and inhibitors of translation. Cell inclusions. Endospores.
- Microbial growth and microbial growth control. Principles of microbial nutrition. Liquid and solid media, growth conditions. Methods for sterilization. Disinfectants and antiseptics.
- Nutrition and metabolism. Overview of metabolism. Fermentation and substrate level phosphorylation. Aerobic and anaerobic respiration. Chemiolithotrophy.
- Microbial genetics. Mutations and mutants. Horizontal gene transfer mechanisms: Transformation, Conjugation Transduction.
- Antibiotics. Structure and mechanisms of action. Minimal inhibitory concentration (MIC). Overview of resistance mechanisms.
TEACHING UNI: VIROLOGY (2 CFU - 16 ore)
- Introduction to Virology: what is a virus? Structure and classification of viruses. Laboratory methods to study the infectious cycle. Biocontainment laboratories (BSL1 to BSL4).
- Viral replication cycles. Attachment and entry. Gene expression and genome replication. The Baltimore classification. Assembly and egress.
- Virus-host interaction. Mechanisms of pathogenesis. Viral oncogenesis.
- Viral diseases and antiviral drugs. AIDS and retroviral drugs. Herpes viruses and anti-herpetic drugs. Viral hepatitis and antivirals targeting HBV and HCV. Respiratory viral infections: influenza and Covid-19.
TEACHING UNIT: HYGIENE (2 CFU - 16 hours)
- Introduction to Hygiene. Health and diseases. Public health. Demographic studies.
- Descriptive epidemiology: measures of frequency, ratios, proportions, crude and adjusted rates. Disease prevalence and incidence.
- Anaytic and experimental epidemiology. Causality criteria. Cross-sectional vs longitudinal studies. Cohort and case-control studies. Relative risk and odds ratio. Clinical trials and preventive interventions.
- Preventive medicine and promotion of health. Primary, secondary and tertiary prevention. Mass screening campaigns. Sensitivity and specificity of screening tests. Oncological screening programs in Italy.
- General epidemiology of infectious diseases. The epidemiological transition. Koch's postulates. The chain of infection. Sources and reservoirs of infection. Modes of transmission. Patterns of infectious disease occurence: endemic, epidemic and pandemic diseases.
- Prophylaxis measures in infectious disease. Direct and indirect prophylaxis: notification, quarantine measures, diagnostic ascertainment, health surveillance, epidemiologic survey, disinfection, disinfestation, sterilization.
- The immune response: anatomical and physical barriers. Innate and adaptive immune responses. Humoral and cellular immune response. The role of B and T lymphocytes and antibodies.
- History of Vaccines. Vaccine-preventable diseases. Vaccine types: life-attenuated vaccines; inactivated vaccines; toxoid vaccines; subunit , recombinant, polysaccharide, and conjugate vaccines. Adjuvants. Administration modes, side effects and contraindications. National vaccine plan. Passive immunization and immune-prophylaxis.
- Epidemiology and prophylaxis of transmittable disease: airborne infectious diseases; sexually-transmitted diseases; chronic viral hepatitis; diseases transmitted via oral-fecal route; vector-borne infectious diseases.
Prerequisites for admission
Mandatory Exams: Physiology and applied biology
Knowledge in organic chemistry and general biology is required.
Knowledge in organic chemistry and general biology is required.
Teaching methods
Lectures. Attendance is highly recommended. A PDF copy of the lecture slides will be made available to the students.
Teaching Resources
- G Dehò, E Galli. Biologia dei microrganismi. Casa Editrice Ambrosiana, 3rd edition (2018)
- N.J. DImmock, A. J. Easton e K. N. Leppard. Introduction to modern virology. 8th edizione (2016)
- M. Pontello, F. Auxilia. Igiene, medicina preventiva e salute globale. Piccin-Nuova Libraria (2022)
- Lecture notes and slides
- N.J. DImmock, A. J. Easton e K. N. Leppard. Introduction to modern virology. 8th edizione (2016)
- M. Pontello, F. Auxilia. Igiene, medicina preventiva e salute globale. Piccin-Nuova Libraria (2022)
- Lecture notes and slides
Assessment methods and Criteria
The exam is written and consists of multiple choice and/or open questions. Maximal score related to complexity of questions. Final score is the sum of scores obtained in the different sections of the exam. The exam, whenever possible, will be held face to face. In case face to face mode will not be possible because of the Covid-19 outbreak the exam will be written and held using the Microsoft Teams platform.
The exam will evaluate:
- the ability of the student to reach course objectives in terms of knowledge and understanding
- the ability of the student to apply knowledge and understanding
- for open questions, the quality of the writing, also in terms of the ability of the student to use the correct terminology in the field of Microbiology and to discuss the different topics in a clear and logic way
The exam will evaluate:
- the ability of the student to reach course objectives in terms of knowledge and understanding
- the ability of the student to apply knowledge and understanding
- for open questions, the quality of the writing, also in terms of the ability of the student to use the correct terminology in the field of Microbiology and to discuss the different topics in a clear and logic way
APPLIED MICROBIOLOGY
BIO/19 - MICROBIOLOGY
MED/42 - HYGIENE AND PUBLIC HEALTH
MED/42 - HYGIENE AND PUBLIC HEALTH
Lessons: 48 hours
Professors:
De Francesco Raffaele, Martorana Alessandra Maria
HYGIENE
BIO/19 - MICROBIOLOGY
MED/42 - HYGIENE AND PUBLIC HEALTH
MED/42 - HYGIENE AND PUBLIC HEALTH
Lessons: 32 hours
Professor:
De Francesco Raffaele
Linea MZ
Responsible
Lesson period
year
Course syllabus
Applied Microbiology Unit (32 ore)
1) Overview of microbiology and its applications.
2) Overview of cell structure: prokaryotes and eukaryotes. Bacteria and Archaea. Cytoplasmic membrane, structure and function. Bacterial transport systems. Cell wall of prokaryotes. Inhibitors of cell wall biogenesis. Outer membrane of Gram-negative bacteria. Capsules. Flagella and bacterial motility. Surface structure for adhesion: pili. The nucleoid. Structure and organization of bacterial chromosome. Inhibitors of DNA replication. Transcription and inhibitors. Cytoplasm. Ribosomes and inhibitors of translation. Cell inclusions. Endospores.
3) Microbial growth and microbial growth control. Principles of microbial nutrition. Liquid and solid media, growth conditions. Methods for sterilization. Disinfectants and antiseptics.
4) Nutrition and metabolism. Overview of metabolism. Fermentation and substrate level phosphorylation. Aerobic and anaerobic respiration. Chemiolithotrophy.
5) Microbial genetics. Mutations and mutants. Horizontal gene transfer mechanisms: Transformation, Conjugation Transduction.
6) Antibiotics. Structure and mechanisms of action. Minimal inhibitory concentration (MIC). Overview on resistance mechanisms.
Virology Unit (16 ore)
1) Introduction to Virology: different types of viral genomes and virions structures
2) The Baltimore Virus Classification. General introduction to viral replication
3) Replication of DNA viruses. Universal rules of DNA replication. Structure of the different types of DNA viral genomes. Replication of dsDNA and ssDNA viruses. Host-pathogen interactions
4) Replication of RNA viruses. Structure of the different types of RNA viral genomes. Replication of ssRNA+ viruses. Replication of ssRNA- viruses. Replication of dsRNA viruses. Replication and structure of Retrovirus.
5) Viral Evolution. Drivers of di Viral Evolution. Differences in viral evolution between DNA and RNA viruses. Viral Quasispecies. Antigenic Drift and antigenic Shift. Bases of phylogenetic analysis of viruses. Specific examples of viral evolution (SarS-CoV2, HIV, Influenza)
6) Discovery and origin of HIV. Structure and replication of HIV. Antiretroviral therapy and drug resistance. HIV pathogenesis and latency
7) Replication cycle HCV e HBV. HCV e HBV antiviral therapy. Pathogenesis of viral induced hepatitis
8) Replication cycle of Herpes viruses and Influenza virus and antiviral therapy
Hygiene Unit (32 ore)
1) Human-Microbe interactions. The human normal microbiota. Microbiota and microbiome.
2) Microbes and disease. Pathogenicity and virulence. Virulence factors.
3) Lines of defenses against bacterial infections: overview of the innate immune system.
4) Pathogens of gastrointestinal tract and respiratory apparatus: mechanism of virulence.
5) Prophylaxis of infectious diseases. Antibiotics and resistance mechanisms. Vaccines.
1) Overview of microbiology and its applications.
2) Overview of cell structure: prokaryotes and eukaryotes. Bacteria and Archaea. Cytoplasmic membrane, structure and function. Bacterial transport systems. Cell wall of prokaryotes. Inhibitors of cell wall biogenesis. Outer membrane of Gram-negative bacteria. Capsules. Flagella and bacterial motility. Surface structure for adhesion: pili. The nucleoid. Structure and organization of bacterial chromosome. Inhibitors of DNA replication. Transcription and inhibitors. Cytoplasm. Ribosomes and inhibitors of translation. Cell inclusions. Endospores.
3) Microbial growth and microbial growth control. Principles of microbial nutrition. Liquid and solid media, growth conditions. Methods for sterilization. Disinfectants and antiseptics.
4) Nutrition and metabolism. Overview of metabolism. Fermentation and substrate level phosphorylation. Aerobic and anaerobic respiration. Chemiolithotrophy.
5) Microbial genetics. Mutations and mutants. Horizontal gene transfer mechanisms: Transformation, Conjugation Transduction.
6) Antibiotics. Structure and mechanisms of action. Minimal inhibitory concentration (MIC). Overview on resistance mechanisms.
Virology Unit (16 ore)
1) Introduction to Virology: different types of viral genomes and virions structures
2) The Baltimore Virus Classification. General introduction to viral replication
3) Replication of DNA viruses. Universal rules of DNA replication. Structure of the different types of DNA viral genomes. Replication of dsDNA and ssDNA viruses. Host-pathogen interactions
4) Replication of RNA viruses. Structure of the different types of RNA viral genomes. Replication of ssRNA+ viruses. Replication of ssRNA- viruses. Replication of dsRNA viruses. Replication and structure of Retrovirus.
5) Viral Evolution. Drivers of di Viral Evolution. Differences in viral evolution between DNA and RNA viruses. Viral Quasispecies. Antigenic Drift and antigenic Shift. Bases of phylogenetic analysis of viruses. Specific examples of viral evolution (SarS-CoV2, HIV, Influenza)
6) Discovery and origin of HIV. Structure and replication of HIV. Antiretroviral therapy and drug resistance. HIV pathogenesis and latency
7) Replication cycle HCV e HBV. HCV e HBV antiviral therapy. Pathogenesis of viral induced hepatitis
8) Replication cycle of Herpes viruses and Influenza virus and antiviral therapy
Hygiene Unit (32 ore)
1) Human-Microbe interactions. The human normal microbiota. Microbiota and microbiome.
2) Microbes and disease. Pathogenicity and virulence. Virulence factors.
3) Lines of defenses against bacterial infections: overview of the innate immune system.
4) Pathogens of gastrointestinal tract and respiratory apparatus: mechanism of virulence.
5) Prophylaxis of infectious diseases. Antibiotics and resistance mechanisms. Vaccines.
Prerequisites for admission
Mandatory Exams: Physiology and applied biology
Knowledge in organic chemistry and general biology is required
Knowledge in organic chemistry and general biology is required
Teaching methods
Lessons and classroom exercises (please refer to the section teaching in covid-19 outbreak)
Teaching Resources
Galli-Dehò Biologia dei Microrganismi Casa Editrice Ambrosiana, 3ed 2018
Scientific papers on e-learning platform
Slides on e-learning platform
Scientific papers on e-learning platform
Slides on e-learning platform
Assessment methods and Criteria
The exam is written and consists of multiple choice and open questions. Maximal score related to complexity of questions. Final score is the sum of scores obtained in the different sections of the exam
The exam, whenever possible, will be held face to face. In case face to face mode will not be possible because of the covid outbreak the exam will be written and held using the Microspft teams platform.
The exam will evaluate
- The ability of the student to reach course objectives in terms of knowledge and understanding
- The ability of the student to apply knowledge and understanding
- The ability of the student to use the correct terminology in the field of Microbiology and to discuss the different topics in a clear and rational way
The exam, whenever possible, will be held face to face. In case face to face mode will not be possible because of the covid outbreak the exam will be written and held using the Microspft teams platform.
The exam will evaluate
- The ability of the student to reach course objectives in terms of knowledge and understanding
- The ability of the student to apply knowledge and understanding
- The ability of the student to use the correct terminology in the field of Microbiology and to discuss the different topics in a clear and rational way
APPLIED MICROBIOLOGY
BIO/19 - MICROBIOLOGY
MED/42 - HYGIENE AND PUBLIC HEALTH
MED/42 - HYGIENE AND PUBLIC HEALTH
Lessons: 48 hours
Professors:
Manganaro Lara, Polissi Alessandra
HYGIENE
BIO/19 - MICROBIOLOGY
MED/42 - HYGIENE AND PUBLIC HEALTH
MED/42 - HYGIENE AND PUBLIC HEALTH
Lessons: 32 hours
Professor:
Polissi Alessandra
Professor(s)
Reception:
Upon request
Department of Pharmacological and Biomolecular Sciences - Via Balzaretti 9 - Milano