Molecular Genetics
A.Y. 2018/2019
Learning objectives
This course aims to provide an in depth understanding of the fundamental principles guiding molecular genetics approaches in either model organisms or human research. By the end of this course students will be able to critically evaluate practical solutions to complex genetic problems by applying the principles of transmission genetics.
Expected learning outcomes
Undefined
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Course syllabus
1) Complex genomes and hereditability of complex traits
Genetic and physical maps: DNA polymorphisms as markers.
Positional cloning, from phenotype to gene: Duchenne muscular dystrophy as a case study. Genetic, cytogenetic and physical mapping, cloning and expression analysis of DMD gene and its mutations, clinical applications.
Functional genomics, from sequence to gene function. Arabidopsis as model system for plant biology. Tools for functional genomics. Sensitised genetic background screens. Quantitative effect of mutations, molecular basis of genetic dominance. Genome-enabled genetics and genome wide association mapping.
Research approaches to nutrigenomics, genomic imprinting and examples of nutriepigenetics.
2) The genetics of differentiation
Sex determination in Drosophila as multilevel regulatory system.
Sex determination in mammals. Role of SRY and SOX9. Mutations determining "sex reversal".
Dosage compensation: hyperactivation and inactivation of chromosome X.
Genetic dissection of the signal transduction determining the transition from vegetative to reproductive phase.
In addition, every year a monographic topic will be proposed.
Reference Material
Teaching slides presented during the course and original research papers/reviews for further reading.
Prerequisites and exam evaluation
An intermediate level of understanding of Mendellian genetics and molecular biology is mandatory.
Evaluation: Final written exam including open question and multiple choices tests.
Recommended Prerequisites
According to the rules of the graduate programme in "Molecular Biology of the Cell".
Teaching methods
Lectures
Language of instruction
English
Program information
Please refer to http://ariel.unimi.it/
WEB Pages
http://ariel.unimi.it/
Genetic and physical maps: DNA polymorphisms as markers.
Positional cloning, from phenotype to gene: Duchenne muscular dystrophy as a case study. Genetic, cytogenetic and physical mapping, cloning and expression analysis of DMD gene and its mutations, clinical applications.
Functional genomics, from sequence to gene function. Arabidopsis as model system for plant biology. Tools for functional genomics. Sensitised genetic background screens. Quantitative effect of mutations, molecular basis of genetic dominance. Genome-enabled genetics and genome wide association mapping.
Research approaches to nutrigenomics, genomic imprinting and examples of nutriepigenetics.
2) The genetics of differentiation
Sex determination in Drosophila as multilevel regulatory system.
Sex determination in mammals. Role of SRY and SOX9. Mutations determining "sex reversal".
Dosage compensation: hyperactivation and inactivation of chromosome X.
Genetic dissection of the signal transduction determining the transition from vegetative to reproductive phase.
In addition, every year a monographic topic will be proposed.
Reference Material
Teaching slides presented during the course and original research papers/reviews for further reading.
Prerequisites and exam evaluation
An intermediate level of understanding of Mendellian genetics and molecular biology is mandatory.
Evaluation: Final written exam including open question and multiple choices tests.
Recommended Prerequisites
According to the rules of the graduate programme in "Molecular Biology of the Cell".
Teaching methods
Lectures
Language of instruction
English
Program information
Please refer to http://ariel.unimi.it/
WEB Pages
http://ariel.unimi.it/
Website
BIO/18 - GENETICS - University credits: 6
Lessons: 48 hours
Professors:
Conti Lucio, Petroni Katia, Tonelli Chiara
Professor(s)
Reception:
upon request of an appointment