Chemical and Toxicological Analysis 2

A.Y. 2020/2021
11
Max ECTS
120
Overall hours
SSD
CHIM/08
Language
Italian
Learning objectives
The aim of the course is to indroduce students with the basic knowledge of the most important analytical techniques, instruments like GC and HPLC in particular, both from a theoretical and practical point of view.
Know the problems related to sampling and treatment of samples to be prepared for analytical procedures to avoid interferences, how we treated in Chemical and Toxycological Analysis 1.
We focused to see which chemical-phisycal methods were the most suitable among:
-UV-vis
-IR
-MS
These skills, in general terms, were increased through ongoing exercises, consisting of multiple choice questions (in relation to topics covered).
The basic theoretical aspects are treated in the 1st Module; in the 2nd Module the laboratory tutorials are carried out
At the end of each laboratory exercise students delivered oral and written experiences descriptions to stimulate their observation and critical skills.
Expected learning outcomes
The student will be able to critically discuss most common qualitative and quantitative analytical techniques, including classic instrumental methods. He will also acquire knowledge and skills required to:
· solve the problems associated with sampling and treatment of samples
· select suitable analytical techniques to solve a given analytical problem
· describe an analytical procedure and correctly report an analytical result
· organize and carry out laboratory activities following high quality and safety standards.
Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
If necessary, the lessons will take place on Microsoft Teams in synchronous mode, at established times, and will be recorded and available on the platform itself in asynchronous mode. Information on how to access Microsoft Teams lessons and other instructional instructions will be uploaded to the course's ARIEL website: it is recommended to consult it regularly. In the event that, taking into account the evolution of the epidemic and compliance with the relevant regulations, it should be possible to carry out activities in the classroom, students will be promptly informed via ARIEL. In any case, the lessons will be recorded and available online. As for the laboratories, they will be partly via video and in part in presence in the lab, depending on the pandemia situation. Information will be promptly available on ARIEL.
Course syllabus
INTRODUCTION

OPTICAL ANALYSIS TECHNIQUES
· Reflectance
· Refractometry
Polarimetry
Turbidimetry

2. INSTRUMENTAL METHODS:
· Infrared spectroscopy
· References to UV-VIS spectroscopy
· Fluorescence spectroscopy
· Phosphorescence spectroscopy
· Laser

3. MASS SPECTROMETRY


4. CHROMATOGRAPHIC TECHNIQUES

· Liquid-solid and liquid-liquid chromatography
· Ion exchange chromatography
· Affinity chromatography
· Filtration gel chromatography
· Column chromatography
· High performance chromatography (HPLC)
· Gas Chromatography (GC)

SAMPLE PREPARATION

· Partition coefficient
· Solvent extraction
· Extraction with supercritical fluids
· Solid phase extraction (SPE, SPME)

DEVELOPMENT AND VALIDATION OF THE ANALYTICAL METHOD

· Guidelines for validation

The topics covered in Module 1 of the course will be deepened in Module 2 with practical exercises in the laboratory.
Prerequisites for admission
No prior knowledge is required, without prejudice to the prerequisites required by the study course.
Teaching methods
Module 1 is structured into a theoretical section (lectures: 6 ECTS - 56 hours) and a practical section (classroom exercises: 1 ECTS, 16 hours).

Module 2 is structured in a practical section (laboratory exercises: 4 credits, 48 ​​hours).
Teaching Resources
· Cozzi R., Protti P., RuaroT.-ANALISI CHIMICA STRUMENTALE
Voll. A, B, C Ed. Zanichelli 1997

· BauerH. H., Christian G. D., O'Relly J. E.-ANALISI STRUMENTALE
Ed. Piccin
Assessment methods and Criteria
The exam is formed by one oral examination (two questions, 15-30 min)
Module 1
CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 7
Lessons: 56 hours
Professor: Arnoldi Anna
Module 2
CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 4
Practicals: 16 hours
Single bench laboratory practical: 48 hours