Chemical and Toxicological Analysis 2

A.Y. 2021/2022
11
Max ECTS
120
Overall hours
SSD
CHIM/08
Language
Italian
Learning objectives
The main objective of this integrated teaching is to introduce students to the knowledge of the most important instrumental analytical techniques used in the chemical, toxicological and environmental fields, both from a theoretical and practical point of view. For this purpose, the lectures are complemented by classroom exercises and practical laboratories.
Students will acquire theoretical and practical skills related to sampling and processing of samples to prepare them for analytical procedures. They will know the following instrumental analytical techniques: UV-visible analysis, atomic absorption and emission, infrared and Raman spectroscopy, gas chromatography, high performance liquid chromatography, supercritical fluid chromatography, capillary electrophoresis, mass spectrometry and hyphenate techniques, such as GC / MS and HPLC / MS. Depending on the type of sample, they will also be able to choose the correct method to implement the qualitative and quantitative analytical protocol. As far as possible, the student will also have the opportunity to personally verify in the laboratory what has been theoretically acquired.
Expected learning outcomes
The student will be able to critically discuss the most common qualitative and quantitative analytical techniques, in particular instrumental methods. He will also acquire the knowledge and skills necessary to: a) solve the problems associated with sampling and handling of samples; b) select suitable analytical techniques to solve a given analytical problem; c) describe an analytical procedure and correctly report an analytical result; d) organize and carry out laboratory activities following high quality and safety standards
Course syllabus and organization

Single session

Responsible
Lesson period
year
More specific information on the delivery modes of training activities for academic year 2021-22 will be provided over the coming months based on the evolution of the public health situation
Course syllabus
INTRODUCTION

OPTICAL ANALYSIS TECHNIQUES
· Reflectance
· Refractometry
Polarimetry
Turbidimetry

INSTRUMENTAL METHODS:
· Infrared spectroscopy
· References to UV-VIS spectroscopy
· Fluorescence spectroscopy
· Phosphorescence spectroscopy
· Laser

MASS SPECTROMETRY


CHROMATOGRAPHIC TECHNIQUES

· Liquid-solid and liquid-liquid chromatography
· Ion exchange chromatography
· Affinity chromatography
· Filtration gel chromatography
· Column chromatography
· High performance chromatography (HPLC)
· Gas Chromatography (GC)

CAPILLARY ELECTROFORESIS

SAMPLE PREPARATION

· Partition coefficient
· Solvent extraction
· Extraction with supercritical fluids
· Solid phase extraction (SPE, SPME)

DEVELOPMENT AND VALIDATION OF THE ANALYTICAL METHOD

· Guidelines for validation

The topics covered in Module 1 of the course will be deepened in Module 2 with practical exercises in the laboratory.
Prerequisites for admission
No prior knowledge is required, with the exception of the prerequisites required by the study course, i.e. Chemical Toxicological Analysis 1 and Organic Chemistry
Teaching methods
Module 1 is structured into a theoretical section (lectures: 6 ECTS - 56 hours) and a practical section (classroom exercises: 1 ECTS, 16 hours).

Module 2 is structured in a practical section (laboratory exercises: 4 credits, 48 ​​hours).
Teaching Resources
Cozzi, Protti, Ruaro
Elementi di chimica analitica strumentale - Tecniche di analisi per Chimica e materiali
terza edizione, 2020, Zanichelli
Assessment methods and Criteria
Multiple choice written test consisting of a series of simple questions covering the whole program (time 30 min).
CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 11
Practicals: 16 hours
Single bench laboratory practical: 48 hours
Lessons: 56 hours
Educational website(s)