Experimental Nutrition

A.Y. 2021/2022
5
Max ECTS
42
Overall hours
SSD
AGR/18
Language
Italian
Learning objectives
The aim of the course is to provide students the principal methodologies applied to nutritional studies, also providing the skills to develop a diet for laboratory animals.
Expected learning outcomes
At the end of the course the student will possess:
-in-depth knowledge of animal nutrition and models applied to nutritional studies;
-knowledge of nutrigenomics and nutri-epigenetics;
-knowledge of laboratory animal diets.
Furthermore the student will be able to
- use and apply the main molecular biology techniques into nutritional studies;
- use in vitro digestion protocols applied to feed and food ;
-design a diet for laboratory animals.
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
The lessons will take place on Microsoft Teams in synchronous mode, following the official calendar, and will be recorded and available on the platform itself in asynchronous mode. The course will alternate different teaching techniques: lectures, seminar method, and recorded laboratory lessons. Lessons may also include pre-recorded materials.
Course syllabus
ATTENDING STUDENTS
Prof. Pinotti
Lectures (12h)
- lab animal nutrition in biomedicine research: how to formulate experimental diets
- Methods in nutritional studies
-Non conventional feed
-Former food / Food waste / ecofeed
-Insects
- Methods for nutritional and functional evaluation

Practical training: 12h
-Methods for characterization of new ingredients
-In vitro feed/food digestibility
-Database consultation
-Technical visits

Prof. Giromini
Lectures (6h)
-Characterization of the main in vitro models applied to nutritional studies
-Application of static and dynamic digestion and absorption models
-Bioactive and functional molecules in the diet
-Epigentics applied to nutrition

Practical training: 12h
-Molecular biology techniques applied to nutritional studies

NON-ATTENDING STUDENTS
Prof. Pinotti
Lectures (12h)
- lab animal nutrition in biomedicine research: how to formulate experimental diets
- Methods in nutritional studies
-Non conventional feed
-Former food / Food waste / ecofeed
-Insects
- Methods for nutritional and functional evaluation

Practical training: 12h
-Methods for characterization of new ingredients
-In vitro feed/food digestibility
-Database consultation
-Technical visits

Prof. Giromini
Lectures (6h)
-Characterization of the main in vitro models applied to nutritional studies
-Application of static and dynamic digestion and absorption models
-Bioactive and functional molecules in the diet
-Epigentics applied to nutrition

Practical training: 12h
-Molecular biology techniques applied to nutritional studies
Prerequisites for admission
n/a
Teaching methods
The course includes both Theoretical and practical classess.
Seminars and educational visits into industries will complete the course activities
Teaching Resources
Giromini, C., Cheli, F., Rebucci, R., & Baldi, A. (2019). Invited review: Dairy proteins and bioactive peptides: Modeling digestion and the intestinal barrier. Journal of dairy science, 102(2), 929-942.

Giromini, C., Lovegrove, J. A., Givens, D. I., Rebucci, R., Pinotti, L., Maffioli, E., ... & Baldi, A. (2019). In vitro-digested milk proteins: Evaluation of angiotensin-1-converting enzyme inhibitory and antioxidant activities, peptidomic profile, and mucin gene expression in HT29-MTX cells. Journal of dairy science, 102(12), 10760-10771.

Baldi et al.,
Biological effects of milk proteins and their peptides with emphasis on those related to the gastrointestinal ecosystem. Journal: Journal of Dairy Research / Volume 72 / Issue S1 / August 2005

Pinotti L., & A. Baldi, et al., Nutritional evaluation of former food products (ex-food) intended for pig nutrition, Food Additives & Contaminants: Part A
doi.org/10.1080/19440049.2017.1306884

Pinotti, L., Ottoboni, M., Luciano, A., Savoini, G., Cattaneo, D., & Tretola, M. (2019). Ex-food in animal nutrition: potentials and challenges. In EAAP Scientific Series (p. 1064580). Wageningen Academic Publishers.

Insects as a source of nutrients: Beyond the Western prejudices
in Animal frontiers Volume 5 Issue 2, April 2015

Nutrient Requirements of Laboratory Animals,
Fourth Revised Edition, 1995 (1995)
Assessment methods and Criteria
Oral exam
critical and in-depth analysis of a topic presented and discussed during the course
AGR/18 - ANIMAL NUTRITION AND FEEDING - University credits: 5
Practicals: 24 hours
Lessons: 18 hours