Experimental Medicine
Doctoral programme (PhD)
A.Y. 2022/2023
Study area
Medicine and Healthcare
PhD Coordinator
Experimental medicine develops and applies technology and biological models for studying pathogenic mechanisms in human disease. It involves various disciplines (biochemistry, biology, molecular biology, anatomy, physiology, pathology, immunology, genetics and microbiology/virology) that are providing a rapidly growing understanding of the mechanisms that enable the development of pathological processes. The course aims to acquaint students with the technologies and research methodologies in the area of biomedicine and biotechnology based on in vitro and in vivo experimental models and '-omic' approaches, and on their application to the study of cellular and molecular mechanisms involved in the pathogenesis of human disease. The final objective is the formation of high-level scientists able to combine an understanding of biological processes and pathogenic mechanisms through the application of advanced experimental techniques. The programme's specific objectives are:
1) develop the ability to define problems and devise experiments to solve them in accordance with scientific standards;
2) study biochemical, molecular and cellular mechanisms in physiopathology using advanced technologies;
3) develop the ability to critically assess scientific literature;
4) provide solid, up-to-date knowledge in the specific field;
5) teach how to conduct independent research, and inform on related ethical implications.
1) develop the ability to define problems and devise experiments to solve them in accordance with scientific standards;
2) study biochemical, molecular and cellular mechanisms in physiopathology using advanced technologies;
3) develop the ability to critically assess scientific literature;
4) provide solid, up-to-date knowledge in the specific field;
5) teach how to conduct independent research, and inform on related ethical implications.
Tutte le classi di laurea magistrale - All classes of master's degree
Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano
- Degree course coordinator: Nicoletta Landsberger
[email protected] - Main offices
Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano - Degree course website
http://d-memphd.unimi.it/
| Title | Professor(s) |
|---|---|
| Maladaptive expression of memory as important determinant of Post-Traumatic Disorders
Requirements: The ideal candidate is already expert in most molecular biology techniques. Preferential skills include experience with in vivo experiments, behavioral analyses and pharmacological approaches. |
|
| Combined pharmacological approaches for Regenerative Medicine
Requirements: Motivated students with a strong commitment to basic and translational research are invited to apply. The candidate should have well-developed social skills, a hands-on attitude and be able to work in a team |
|
| New mechanisms controlling macrophages functions: role of MS4A proteins
Requirements: Basic cellular and molecular biology; biochemical competences |
|
| Priming of Mesenchymal Stem/ Stromal Cells to increase their secretome therapeutic potential in counteracting osteoarthritis through EV action
Requirements: Strong scientific motivation with a hardworking attitude. Any experience in cell culture and molecular biology is appreciated together with the skill in problem solving. Strong commitment to basic and translational research and good group communications are welcome. |
|
| Study of the pathogenic mechanisms underlying the aberrant communication of tumor and the surrounding microenvironment
Requirements: Strong motivation, expertise in basic cellular and molecular biology methods, knowledge of cancer cell biology and immune system |
|
| A Multiomic Approach To Dissect Signatures Of Response and Resistance To Immunotherapy Approaches for Large B-Cell Lymphoma | |
| Immunopathogenesis of thymic epithelial tumors and related autoimmune disorders |
S. FRANZESE
|
| New mitochondrial-based therapy to promote muscle regeneration in Duchenne Muscular Dystrophy
Requirements: Students should be interested in the investigation of mitochondrial-dependent pathways involved in the progression of DMD and in the setting of preclinical studies with different DMD models to test the impact of their manipulation. |
|
| Heart and kidney inflammation in obesity: focus on the crosstalk between Dipeptidyl Peptidase-4 (DPP4), Glucagon-Like Peptide-1 Receptor (GLP-1R) and Angiotensin-converting enzyme 2 (ACE2) as risk factors and potential therapeutic targets in COVID-19
Requirements: Knowledge of the main techniques of cellular and molecular biology [cell cultures, gene silencing, gene and protein expression analysis (RT- Real Time PCR, Western blot)], and biochemical assays for the evaluation of biomarkers in fluids [e.g. enzyme immunoassays (ELISA)]. |
|
| Study of the role of IncRNAs and transcriptional dysregulation in the pathogenesis of Congenital Central Hypoventilation Syndrome using patient-derived Induced Pluripotent Stem Cells (iPSCs).
Requirements: Basic cellular and molecular biology techniques (PCR, RNA extraction, RT-qPCR, western blot), mammalian celI culture and immunofluorescence. |
|
| Analysis of GABAergic circuitry in Pcdh19 mouse model of Developmental and epileptic encephalopathy 9 (DEE9) | |
| Evaluation of the diagnostic and prognostic potential of osteoimmunology markers in patients with osteomyelitis
Requirements: Molecular and cellular biology, enzyme immunoassays, real time PCR, evaluation of laboratory medicine parameters |
|
| Identifying and characterizing novel modifier genes for Rett syndrome
Requirements: No specific requirement although previous experience in molecular and/or cellular biology will be an advantage |
|
| Study of the IL7-R+ monocyte subset in infection and tumor settings
Requirements: Basic cellular and molecular biology |
|
| Identification of molecular mechanisms involved in the risk of pediatric multiple sclerosis
Requirements: Experience in DNA, mRNA and stool extraction for gut microbiota; experience in cell culture; ability to maintain clinical and laboratory database |
|
| Targeting the tumor-escape mechanisms from immune-surveillance of metastatic colon rectal cancer.
Requirements: Advanced knowledge/experience on analytical approaches with computational methods and programming language, preferred experience in handling NGS data. Availability of spending periods abroad for training and collaborations. Experience in computational medicine biology will be preferred to develop the analytic and computational part of the project. |
R. PIAZZA
|
| Role of oxidative stress in the vulnerability for psychiatric diseases and in the mechanism of action of psychotropic drugs
Requirements: Curiosity about science, interest in neuroscience and mental health, willingness to get involved and grow in a group through discussion, ask questions and think about how to find answers. Expertise in gene and protein expression analyses, cell culture |
|
| Deciphering the molecular hubs shaping CD4 T regulatory lymphocyte function
Requirements: Molecular biology expertise |
|
| In vivo zebrafish model to dissect novel pathological pathways and potential therapies for congenital highly-disruptive diseases
Requirements: Experience in endocrine regulatory mechanisms of reproduction and/or thyroid development, whole exome sequencing technique and analysis, molecular biology techniques for gene expression studies, zebrafish model |
|
| Molecular mechanisms regulating insulin like growth factor (IGF) system in adrenocortical carcinomas
Requirements: Cell cultures; cell transfection and silencing; protein detection (western blot, co-/immunoprecipitation); nucleic acid extraction, PCR. |
|
| Zebrafish as a tool to study the role of the innate immune system in the pathogenesis of human diseases and identify possible pharmacological treatments
Requirements: Expertise in cellular and molecular biology |
|
| Dissecting nuclear and axonal transport defects in ALS patient-derived iPSC-motoneurons carrying TARDBP and C9orf72 gene mutations |
C. COLOMBRITA
|
| Tumor-associated microbiota as new player in modulating the immune response in the tumor microenvironment and in promoting immunotherapy resistance |
V. LE NOCI
|
| Adipo-NETwork: inside the crosstalk between adipose cells and gastroenteropancreatic neuroendocrine tumors (GEP-NETs)
Requirements: Strong interest for basic and translational research. Previous experience in cell culture and molecular biology is appreciated. |
G. GAUDENZI
|
| Correction of the genetic defect in induced pluripotent stem cell lines by chromosome transplantation
Requirements: The candidate has to demonstrate experience in the main techniques of cell and molecular biology, mammalian cell culture and immunofluorescence assays. Experience in culturing induced pluripotent stem cells (iPS) and in basic cytogenetic techniques (chromosome preparations, karyotype analysis) and basic molecular cytogenetics (FISH) will be considered as a preferential title. |
|
| Surface topography-mediated foreign body response and tumorigenesis: an integrated study on the role of implants’ texturization
Requirements: Basic experience/knowledge of molecular and cellular biology techniques. Knowledge of flow cytometry will be an advantage. Availability of spending periods abroad for training and collaborations. Experience in immunology or medicine biology will be preferred. |
|
| Integrating pharmacogenomic and pharmacometabolomic data towards personalized opioid therapies for cancer pain
Requirements: Strong background in genetics (population genetics, genetic epidemiology), knowledge of molecular genetics and biology methodologies, omics technologies, data analysis, bioinformatics, medical statistics. Strong scientific motivation with positive attitude to work, especially in groups; collaborative attitude and good interpersonal and communication skills. |
|
| Study of the effect of Tumor Educated Macrophages on neural cells (Ex DM 352/2022) |
Courses list
January 2023
| Courses or activities | Professor(s) | ECTS | Total hours | Language |
|---|---|---|---|---|
| Compulsory | ||||
| Improving Your Statistical Skills in Biomedical Sciences | 2 | 10 | English | |
| Quantification and Characterization of Extracellular Vesicles in Biomedical Research | 2 | 10 | English | |
February 2023
| Courses or activities | Professor(s) | ECTS | Total hours | Language |
|---|---|---|---|---|
| Optional | ||||
| Advanced Biomedical Statistics | 2 | 10 | English | |
| Preclinical Models in Oncology | 2 | 10 | English | |
April 2023
| Courses or activities | Professor(s) | ECTS | Total hours | Language |
|---|---|---|---|---|
| Compulsory | ||||
| Preclinical Models in Neuromuscular Disorders | 2 | 10 | English | |
| Optional | ||||
| Supporting Tools for Drug-Target Prioritisation and Analysis of Biological Networks | 2 | 10 | English | |
May 2023
| Courses or activities | Professor(s) | ECTS | Total hours | Language |
|---|---|---|---|---|
| Compulsory | ||||
| Flow Cytometry in Biomedical Research | 4 | 21 | English | |
| Optional | ||||
| Alternative Splicing in Health and Disease | 2 | 10 | English | |
June 2023
| Courses or activities | Professor(s) | ECTS | Total hours | Language |
|---|---|---|---|---|
| Optional | ||||
| Single Cell Sequencing: a Deep Dive Into Dissecting Cell Populations At Single Cell Level | 2 | 10 | English | |
Following the programme of study
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